Department of Pharmacology, Physiology of Animals and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria.
Exp Parasitol. 2012 Nov;132(3):378-82. doi: 10.1016/j.exppara.2012.08.017. Epub 2012 Sep 7.
Interleukin (IL)-12 and IL-10 are immunoregulatory cytokines with an antagonistic effect on the T-helper (Th)1/Th2 cytokine balance and they provide a functional link between innate resistance and the adaptive immune response. This investigation was conducted to determine the expression of IL-10 and IL-12B mRNA levels in chickens' gut mucosa infected with Eimeria tenella and in sulfachlorpyrazine-sodium treated animals after infection. Broiler chickens were randomly allocated in three groups: healthy untreated control; infected untreated animals and infected, treated with sulfachlorpyrazine sodium chickens 6 days after the challenge with an E. tenella. Quantitative real time PCR analysis was performed using specific primer pairs and probes for IL-10 and IL-12B. The expression of IL-10 mRNA was greater in the duodenum then in the caecum and the liver of healthy chickens. E. tenella infection led to significant up-regulation of IL-10 mRNA in the caecum, followed by mRNA in the liver. A significant down regulation was observed mainly in the caecum after the treatment with sulfachlorpyrazine. In contrast, IL-12B expression in all investigated tissues remained insignificantly affected in the studied groups of animals. Distinct up-regulation of IL-10 mRNA, after the challenge with E. tenella, in the caecum can be attributed to the tissue tropism of Eimeria spp. The production of IL-12 is regulated by negative feedback through IL-10 which explains lack of increase in IL-12B mRNA. Sulfonamide treatment resulted in clinical improvement and restoration of IL-10 mRNA to the levels observed in healthy chickens.
白细胞介素 (IL)-12 和 IL-10 是免疫调节细胞因子，对辅助性 T 细胞 (Th)1/Th2 细胞因子平衡具有拮抗作用，它们为先天抵抗和适应性免疫反应之间提供了功能联系。本研究旨在确定感染柔嫩艾美耳球虫后鸡肠道黏膜中 IL-10 和 IL-12B mRNA 水平的表达，以及感染后磺胺氯吡嗪钠处理动物的表达情况。肉鸡被随机分配到三组：健康未处理对照组；未处理感染组和感染后用磺胺氯吡嗪钠处理组，在感染柔嫩艾美耳球虫 6 天后进行处理。使用针对 IL-10 和 IL-12B 的特异性引物对和探针进行定量实时 PCR 分析。在健康鸡的十二指肠中，IL-10 mRNA 的表达高于盲肠和肝脏。感染柔嫩艾美耳球虫导致盲肠中 IL-10 mRNA 显著上调，其次是肝脏。磺胺氯吡嗪处理后，主要在盲肠中观察到显著下调。相比之下，在所研究的动物组中，所有研究组织中的 IL-12B 表达均无明显变化。在感染柔嫩艾美耳球虫后，盲肠中 IL-10 mRNA 的明显上调可能归因于艾美耳球虫属的组织嗜性。IL-12 的产生受 IL-10 的负反馈调节，这解释了 IL-12B mRNA 没有增加的原因。磺胺类药物治疗导致临床症状改善，并将 IL-10 mRNA 恢复到健康鸡观察到的水平。
