Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka Str., 02-018 Warsaw, Poland.
Exp Parasitol. 2010 Sep;126(1):103-5. doi: 10.1016/j.exppara.2010.01.025. Epub 2010 Feb 6.
Trophozoites and cysts of free-living Acanthamoeba castellanii present a serious risk to human health as causative agents of human diseases such as fatal granulomatous amoebic encephalitis and Acanthamoeba keratitis that is reported from various part of the world, also in Poland, with increasing frequency, particularly in the contact lens wearers. The amphizoic amoebae are generally extremely resistant to different chemical agents, however, several strains/isolates within A. castellanii may differ in virulence. Among the features considered as associated with the amoeba pathogenicity, temperature tolerance and resistance to different environmental conditions are reported. In the present study, A. castellanii strain cultured in 26 degrees C after several year passages were tested for sensibility/tolerance to instant temperature changes as well as exposition to deuterium oxide, D2O. Significant decrease of number of viable amoebae during in vitro exposition to D2O occurred, but no changes in trophozoites/cysts ratio. The ability of the strain examined to develop in higher temperature may indicate a wide adaptation reserve and its pathogenic potential.
自由生活的福氏耐格里阿米巴滋养体和包囊是人类健康的严重威胁，是人类疾病的病原体，如致命的肉芽肿性阿米巴脑炎和棘阿米巴角膜炎，这种疾病在世界各地都有报道，在波兰也越来越频繁，尤其是在隐形眼镜佩戴者中。两栖阿米巴通常对各种化学制剂具有极强的抵抗力，然而，福氏耐格里阿米巴内的几个菌株/分离株在毒力上可能存在差异。在被认为与阿米巴致病性相关的特征中，有报道称其对温度耐受性和对不同环境条件的抵抗力。在本研究中，对在 26°C 下培养了数年的福氏耐格里阿米巴株进行了即时温度变化敏感性/耐受性测试，以及对重水（D2O）的暴露测试。在体外暴露于 D2O 过程中，活阿米巴数量显著减少，但滋养体/包囊的比例没有变化。该菌株在较高温度下生长的能力表明其具有广泛的适应储备和潜在的致病性。
