Padzik Marcin, Chomicz Lidia, Szaflik Jacek P, Chruścikowska Agnieszka, Perkowski Konrad, Szaflik Jerzy
Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka Str., 02-018 Warsaw, Poland.
Department of Medical Biology, Medical University of Warsaw, 73 Nowogrodzka Str., 02-018 Warsaw, Poland.
Exp Parasitol. 2014 Nov;145 Suppl:S98-S101. doi: 10.1016/j.exppara.2014.06.014. Epub 2014 Jun 23.
Free-living, cosmopolitan amoebae of the Acanthamoeba genus may be the causative agents of Acanthamoeba keratitis (AK) - a progressive, vision-threatening infection of the human cornea described particularly among contact lens wearers. Use of contact lens care solutions, effective against these organisms, is important in preventing AK infections. 3 different strains of Acanthamoeba castellanii of the T4 genotype (Neff strain and two others, isolated from patients with AK) were exposed to 4 selected multipurpose contact lens care solutions available in Poland: Ciba Vision AoSept Plus, Bausch & Lomb ReNu MultiPlus, Alcon Opti-Free, Ciba Vision Solo Care Aqua. No amoebicidal effect was observed. The strongest amoebostatic effect was visible after 24h of exposition to Opti-Free and ReNu solution and associated with percentage increase of rounded, motionless forms. This is significantly longer than minimum disinfection time recommended by manufacturers of all tested multipurpose solutions. Surprisingly, no clear induction of the encystation process was observed.
棘阿米巴属的自由生活、世界性阿米巴可能是棘阿米巴角膜炎（AK）的病原体，这是一种进行性的、威胁视力的人类角膜感染，尤其在隐形眼镜佩戴者中较为常见。使用对这些生物体有效的隐形眼镜护理液对于预防AK感染很重要。将3种不同的T4基因型卡氏棘阿米巴菌株（Neff菌株和另外两种从AK患者中分离出的菌株）暴露于波兰市场上可供选择的4种多功能隐形眼镜护理液中：爱尔康傲滴恒润、博士伦润明全护理液、爱尔康Opti-Free、爱尔康傲滴清润护理液。未观察到杀阿米巴作用。暴露于Opti-Free和润明护理液24小时后，可见最强的抑阿米巴作用，且与圆形、静止形态的百分比增加有关。这明显长于所有测试的多功能护理液制造商推荐的最短消毒时间。令人惊讶的是，未观察到明显的包囊形成过程诱导。
