单宁酸修饰的银纳米颗粒增强了三种多功能隐形眼镜护理液的抗棘阿米巴活性，且未增加其细胞毒性。
Tannic acid-modified silver nanoparticles enhance the anti-Acanthamoeba activity of three multipurpose contact lens solutions without increasing their cytotoxicity.
作者信息
Hendiger Edyta B, Padzik Marcin, Żochowska Agnieszka, Baltaza Wanda, Olędzka Gabriela, Zyskowska Diana, Bluszcz Julita, Jarzynka Sylwia, Chomicz Lidia, Grodzik Marta, Hendiger Jacek, Piñero José E, Grobelny Jarosław, Ranoszek-Soliwoda Katarzyna, Lorenzo-Morales Jacob
机构信息
Laboratory of Parasitology, Department of Medical Biology, Medical University of Warsaw, 14/16 Litewska Street, 00-575, Warsaw, Poland.
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez S/N, 38203, Tenerife, Spain.
出版信息
Parasit Vectors. 2020 Dec 22;13(1):624. doi: 10.1186/s13071-020-04453-z.
BACKGROUND
Free-living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans that cause a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lens use. Appropriate eye hygiene and effective contact lens disinfection are crucial in the prevention of AK because of the lack of effective therapies against it. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent AK infections. Synthesized nanoparticles (NPs) have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that some plant metabolites, including tannins, have anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of tannic acid-modified silver NPs (AgTANPs) conjugated with selected multipurpose contact lens solutions.
METHODS
The anti-amoebic activities of pure contact lens care solutions, and NPs conjugated with contact lens care solutions, were examined in vitro by a colorimetric assay based on the oxido-reduction of alamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. The results were statistically analysed by ANOVA and Student-Newman-Keuls test using P < 0.05 as the level of statistical significance.
RESULTS
We show that the NPs enhance the anti-Acanthamoeba activities of the tested contact lens solutions without increasing their cytotoxicity profiles. The activities are enhanced within the minimal disinfection time recommended by the manufacturers.
CONCLUSIONS
The conjugation of the selected contact lens solutions with AgTANPs might be a novel and promising approach for the prevention of AK infections among contact lens users.
背景
棘阿米巴属的自由生活阿米巴是世界性分布、广泛存在的原生动物，可引发一种严重的、威胁视力的角膜感染，即棘阿米巴角膜炎（AK）。越来越多的AK病例大多与隐形眼镜的使用有关。由于缺乏针对AK的有效治疗方法，适当的眼部卫生和有效的隐形眼镜消毒对于预防AK至关重要。目前可用的多功能隐形眼镜消毒系统对棘阿米巴滋养体和包囊并不完全有效。迫切需要提高这些系统的消毒活性以预防AK感染。合成纳米颗粒（NPs）最近已被研究并被提议作为新一代抗菌剂。还已知一些植物代谢产物，包括单宁，具有抗寄生虫活性。本研究的目的是评估与选定的多功能隐形眼镜护理液结合的单宁酸修饰银纳米颗粒（AgTANPs）的抗阿米巴活性和细胞毒性。
方法
通过基于alamarBlue氧化还原的比色法在体外检测纯隐形眼镜护理液以及与隐形眼镜护理液结合的NPs的抗阿米巴活性。使用成纤维细胞HS-5（ATCC CRL-11882）细胞系进行细胞毒性测定。结果采用方差分析和Student-Newman-Keuls检验进行统计学分析，以P < 0.05作为统计学显著性水平。
结果
我们表明，纳米颗粒增强了所测试的隐形眼镜护理液的抗棘阿米巴活性，而不增加其细胞毒性。在制造商推荐的最短消毒时间内，活性得到增强。
结论
选定的隐形眼镜护理液与AgTANPs结合可能是预防隐形眼镜使用者发生AK感染的一种新颖且有前景的方法。
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