Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India.
Int J Biol Macromol. 2010 May 1;46(4):445-50. doi: 10.1016/j.ijbiomac.2010.02.001. Epub 2010 Feb 6.
We prepared Polysulfone (PS) globules by pouring the PS solution droplets in to the non-solvent water. PS solutions (10, 15, and 20% (w/v), concentrations) of different viscosities resulted different physical appearances in globules. The lipase immobilizing amount (4.32 mg/g) was maximum for PS 20% globules as it possessed maximum BET pore surface area. The olive oil hydrolytic reaction parameters were fitted into the Lineweaver-Burk plot. The K(m) (apparent) values of all the immobilized globules were higher (83.3mM) with respect to free lipases (62.5mM) where as V(max) (apparent) values followed the reverse trend (129.8 U/mg for 20% and 120.5 U/mg for both 15% and 10% PS immobilized globules and 153.8 U/mg for the free lipase respectively). The hydrolytic activity to olive oil was decreased ( approximately 16-20%) after five cycles.
我们通过将 PS 溶液液滴滴入非溶剂水中来制备聚砜 (PS) 小球。不同粘度的 PS 溶液 (10%、15%和 20%（w/v），浓度) 导致小球呈现出不同的物理外观。PS 20%小球的固定化脂肪酶量（4.32mg/g）最高，因为它具有最大的 BET 孔径表面积。橄榄油水解反应参数拟合到 Lineweaver-Burk 图中。所有固定化小球的 K(m)（表观）值均高于游离脂肪酶（62.5mM）（83.3mM），而 V(max)（表观）值则相反（20% PS 固定化小球为 129.8U/mg，15% 和 10% PS 固定化小球分别为 120.5U/mg，游离脂肪酶为 153.8U/mg）。五次循环后，对橄榄油的水解活性降低（约 16-20%）。
