Aquatic Science Research Unit, Curtin University of Technology, Brodie Hall Building, 1 Turner Avenue, Technology Park, Bentley, Western Australia 6102, Australia.
Fish Shellfish Immunol. 2010 May-Jun;28(5-6):957-60. doi: 10.1016/j.fsi.2010.01.020. Epub 2010 Feb 6.
Six isonitrogenous and isocalorific diets supplemented with five different levels of beta-1,3-glucan (0.08%, 0.1%, 0.2%, 0.4% and 0.8%) were formulated and tested for marron (Cherax tenuimanus) growth, survival, organosomatic indices, osmoregulatory capacity and immunological parameters (total and differential haemocyte counts, haemolymph clotting time and bacteraemia). The sixth diet without any beta-1,3-glucan was used as a control. Each diet was provided to 18 marron (0.47 +/- 0.02 g initial weight) replicated 3 times in individual 250 L fiberglass cylindrical tanks. Each tank was provided with a biological filtration recirculating water system. After 84 days of culture, the survival and yield were higher in the marron fed 0.1% beta glucan supplemented diet. The different levels of beta glucan did not alter any of the physiological parameters of marron. However, dietary supplementation with beta glucan resulted in significantly higher (P < 0.05) total haemocyte count (THC) and granular cells. The bacteraemia rank was lower in all diets having beta glucan supplemented with more than or equal to 0.1% compared to the control and 0.08% beta glucan supplemented diets. Results suggest that dietary beta-1,3-glucan at a minimum concentration of 0.1-0.2% can improve the immune system of marron.
六种等氮等热量的饲料中添加了五种不同水平的β-1,3-葡聚糖（0.08%、0.1%、0.2%、0.4%和0.8%），用于测试螯虾（Cherax tenuimanus）的生长、存活、体器官指数、渗透压调节能力和免疫参数（总血细胞计数和分类计数、血淋巴凝固时间和菌血症）。第六种不含任何β-1,3-葡聚糖的饲料用作对照。每种饲料都提供给 18 只螯虾（初始体重 0.47 +/- 0.02 g），在单独的 250 升玻璃纤维圆柱形水箱中重复 3 次。每个水箱都配备了生物过滤循环水系统。经过 84 天的养殖，摄食 0.1%β-葡聚糖补充饲料的螯虾存活率和产量更高。不同水平的β-葡聚糖没有改变螯虾的任何生理参数。然而，β-葡聚糖的膳食补充显著增加了（P < 0.05）总血细胞计数（THC）和颗粒细胞。与对照组和 0.08%β-葡聚糖补充饲料相比，所有添加了β-葡聚糖的饲料中的菌血症等级都较低，β-葡聚糖的添加量至少为 0.1-0.2%。结果表明，β-1,3-葡聚糖的最低膳食浓度为 0.1-0.2%，可以改善螯虾的免疫系统。
