文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

实验性减毒活人副流感病毒 2 型疫苗在体外人纤毛气道上皮中的生长受限与在非洲绿猴中的减毒作用相平行。

Growth restriction of an experimental live attenuated human parainfluenza virus type 2 vaccine in human ciliated airway epithelium in vitro parallels attenuation in African green monkeys.

机构信息

Laboratory of Infectious Diseases, RNA Viruses Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892-2007, USA.

出版信息

Vaccine. 2010 Mar 24;28(15):2788-98. doi: 10.1016/j.vaccine.2010.01.050. Epub 2010 Feb 20.

DOI:10.1016/j.vaccine.2010.01.050
PMID:20139039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2844349/
Abstract

Human parainfluenza viruses (HPIVs) are common causes of severe pediatric respiratory viral disease. We characterized wild-type HPIV2 infection in an in vitro model of human airway epithelium (HAE) and found that the virus replicates to high titer, sheds apically, targets ciliated cells, and induces minimal cytopathology. Replication of an experimental, live attenuated HPIV2 vaccine strain, containing both temperature sensitive (ts) and non-ts attenuating mutations, was restricted >30-fold compared to rHPIV2-WT in HAE at 32 degrees C and exhibited little productive replication at 37 degrees C. This restriction paralleled attenuation in the upper and lower respiratory tract of African green monkeys, supporting the HAE model as an appropriate and convenient system for characterizing HPIV2 vaccine candidates.

摘要

人副流感病毒（HPIVs）是引起严重小儿呼吸道病毒病的常见原因。我们对人类气道上皮（HAE）体外模型中的野生型 HPIV2 感染进行了特征描述，发现该病毒可高效复制，呈顶端释放，靶向纤毛细胞，并引起最小的细胞病变。与 rHPIV2-WT 相比，含有温度敏感（ts）和非 ts 减毒突变的实验性减毒活 HPIV2 疫苗株在 32°C 的 HAE 中复制受到 >30 倍的限制，在 37°C 时几乎没有有效复制。这种限制与非洲绿猴上呼吸道和下呼吸道的衰减相平行，支持 HAE 模型作为一种合适且方便的系统，用于鉴定 HPIV2 疫苗候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/ee510c18c201/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/2cd813589959/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/d549db43d764/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/b8932edd43d7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/7cd8142d3baa/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/090c1775a2a3/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/ee510c18c201/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/2cd813589959/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/d549db43d764/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/b8932edd43d7/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/7cd8142d3baa/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/090c1775a2a3/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ac8/7126223/ee510c18c201/gr6.jpg

相似文献

[1]
Growth restriction of an experimental live attenuated human parainfluenza virus type 2 vaccine in human ciliated airway epithelium in vitro parallels attenuation in African green monkeys.

Vaccine. 2010-2-20

[2]
Role of interferon in the replication of human parainfluenza virus type 1 wild type and mutant viruses in human ciliated airway epithelium.

J Virol. 2008-8

[3]
Human parainfluenza virus type 2 V protein inhibits interferon production and signaling and is required for replication in non-human primates.

Virology. 2009-12-7

[4]
Recombinant human parainfluenza virus type 2 vaccine candidates containing a 3' genomic promoter mutation and L polymerase mutations are attenuated and protective in non-human primates.

Vaccine. 2007-8-21

[5]
Evaluation of a Live-Attenuated Human Parainfluenza Virus Type 2 Vaccine in Adults and Children.

J Pediatric Infect Dis Soc. 2023-4-18

[6]
Cold-passaged human parainfluenza type 3 viruses contain ts and non-ts mutations leading to attenuation in rhesus monkeys.

Virus Res. 1992-3

[7]
Comparison of mutations in human parainfluenza viruses during passage in primary human bronchial/tracheal epithelial air-liquid interface cultures and cell lines.

Microbiol Spectr. 2024-9-3

[8]
Replacement of the ectodomains of the hemagglutinin-neuraminidase and fusion glycoproteins of recombinant parainfluenza virus type 3 (PIV3) with their counterparts from PIV2 yields attenuated PIV2 vaccine candidates.

J Virol. 2000-7

[9]
Enhanced growth of influenza A virus by coinfection with human parainfluenza virus type 2.

Med Microbiol Immunol. 2016-6

[10]
A live attenuated recombinant chimeric parainfluenza virus (PIV) candidate vaccine containing the hemagglutinin-neuraminidase and fusion glycoproteins of PIV1 and the remaining proteins from PIV3 induces resistance to PIV1 even in animals immune to PIV3.

Vaccine. 2000-1-31

引用本文的文献

[1]
Mutations in the F protein of the live-attenuated respiratory syncytial virus vaccine candidate ΔNS2/Δ1313/I1314L increase the stability of infectivity and content of prefusion F protein.

PLoS One. 2024

[2]
Leveraging 3D Model Systems to Understand Viral Interactions with the Respiratory Mucosa.

Viruses. 2020-12-11

[3]
Isolation and characterization of WUPyV in polarized human airway epithelial cells.

BMC Infect Dis. 2020-7-9

[4]
Effect of genetic background and delivery route on the preclinical properties of a live attenuated RSV vaccine.

PLoS One. 2018-6-19

[5]
Respiratory Syncytial Virus Uses CX3CR1 as a Receptor on Primary Human Airway Epithelial Cultures.

PLoS Pathog. 2015-12-11

[6]
Preventing Cleavage of the Respiratory Syncytial Virus Attachment Protein in Vero Cells Rescues the Infectivity of Progeny Virus for Primary Human Airway Cultures.

J Virol. 2015-11-18

[7]
Human airway epithelial cell cultures for modeling respiratory syncytial virus infection.

Curr Top Microbiol Immunol. 2013

[8]
Human parainfluenza virus serotypes differ in their kinetics of replication and cytokine secretion in human tracheobronchial airway epithelium.

Virology. 2012-9-7

[9]
Pathogenesis of acute respiratory illness caused by human parainfluenza viruses.

Curr Opin Virol. 2012-3-3

[10]
Comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (W3A), parainfluenza virus type 3, and respiratory syncytial virus.

Virology. 2011-10-8

本文引用的文献

[1]
Human parainfluenza virus type 2 V protein inhibits interferon production and signaling and is required for replication in non-human primates.

Virology. 2009-12-7

[2]
CFTR delivery to 25% of surface epithelial cells restores normal rates of mucus transport to human cystic fibrosis airway epithelium.

PLoS Biol. 2009-7

[3]
Avian Influenza virus glycoproteins restrict virus replication and spread through human airway epithelium at temperatures of the proximal airways.

PLoS Pathog. 2009-5

[4]
Efficacy and safety of 1 and 2 doses of live attenuated influenza vaccine in vaccine-naive children.

Pediatr Infect Dis J. 2009-5

[5]
Parainfluenza virus infection of young children: estimates of the population-based burden of hospitalization.

J Pediatr. 2009-5

[6]
Human parainfluenza virus type 1 C proteins are nonessential proteins that inhibit the host interferon and apoptotic responses and are required for efficient replication in nonhuman primates.

J Virol. 2008-9

[7]
Measles virus blind to its epithelial cell receptor remains virulent in rhesus monkeys but cannot cross the airway epithelium and is not shed.

J Clin Invest. 2008-7

[8]
Role of interferon in the replication of human parainfluenza virus type 1 wild type and mutant viruses in human ciliated airway epithelium.

J Virol. 2008-8

[9]
Select paramyxoviral V proteins inhibit IRF3 activation by acting as alternative substrates for inhibitor of kappaB kinase epsilon (IKKe)/TBK1.

J Biol Chem. 2008-5-23

[10]
The pathology of influenza virus infections.

Annu Rev Pathol. 2008

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索