比较副流感病毒 5（W3A）、副流感病毒 3 型和呼吸道合胞病毒在人呼吸道上皮细胞中的不同细胞病变效应。
Comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (W3A), parainfluenza virus type 3, and respiratory syncytial virus.
机构信息
Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
出版信息
Virology. 2011 Dec 5;421(1):67-77. doi: 10.1016/j.virol.2011.08.020. Epub 2011 Oct 8.
Abstract
Parainfluenza virus 5 (PIV5) infects a wide range of animals including dogs, pigs, cats, and humans; however, its association with disease in humans remains controversial. In contrast to parainfluenza virus 3 (PIV3) or respiratory syncytial virus (RSV), PIV5 is remarkably non-cytopathic in monolayer cultures of immortalized epithelial cells. To compare the cytopathology produced by these viruses in a relevant human tissue, we infected an in vitro model of human ciliated airway epithelium and measured outcomes of cytopathology. PIV5, PIV3 and, RSV all infected ciliated cells, and PIV5 and PIV3 infection was dependent on sialic acid residues. Only PIV5-infected cells formed syncytia. PIV5 infection resulted in a more rapid loss of infected cells by shedding of infected cells into the lumen. These studies revealed striking differences in cytopathology of PIV5 versus PIV3 or RSV and indicate the extent of cytopathology determined in cell-lines does not predict events in differentiated airway cells.
摘要
副流感病毒 5（PIV5）感染范围广泛，包括狗、猪、猫和人类等多种动物；然而，其与人类疾病的关联仍存在争议。与副流感病毒 3（PIV3）或呼吸道合胞病毒（RSV）不同，PIV5 在永生化上皮细胞的单层培养物中表现出显著的非细胞病变性。为了比较这些病毒在相关人类组织中产生的细胞病变，我们感染了体外人纤毛气道上皮模型，并测量了细胞病变的结果。PIV5、PIV3 和 RSV 均可感染纤毛细胞，且 PIV5 和 PIV3 的感染依赖于唾液酸残基。只有 PIV5 感染的细胞形成合胞体。PIV5 感染导致受感染细胞通过将受感染细胞脱落到腔中而更快地丧失。这些研究揭示了 PIV5 与 PIV3 或 RSV 的细胞病变之间的显著差异，并表明细胞系中确定的细胞病变程度不能预测分化气道细胞中的事件。
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