在极化的人呼吸道上皮细胞中分离和鉴定 WUPyV。
Isolation and characterization of WUPyV in polarized human airway epithelial cells.
机构信息
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, 100 Ying-Xin St., Xi-Cheng District, Beijing, 100052, China.
Department of Pediatrics, Beijing Friendship Hospital, Capital Medical University, 95 Yong-An St., Xi-Cheng District, Beijing, 100050, China.
出版信息
BMC Infect Dis. 2020 Jul 9;20(1):488. doi: 10.1186/s12879-020-05224-y.
BACKGROUND
Washington University polyomavirus (WUPyV) is a novel human polyomavirus detected in childwith acute respiratory infection in 2007. However, the relationship between WUPyV and respiratory diseases has yet to be established for lacking of a suitable in vitro culture system.
METHODS
To isolate WUPyV with human airway epithelial (HAE) cells, the positive samples were incubated in HAE, and then the nucleic acid, VP1 protein and virions were detected using real-time PCR, immunofluorescence and electron microscopy respectively.
RESULTS
The result showed that WUPyV could replicate effectively in HAE cells and virions with typical polyomavirus characteristics could be observed. Additionally, the entire genome sequence of the isolated strain (BJ0771) was obtained and phylogenetic analysis indicated that BJ0771 belongs to gene cluster I.
CONCLUSIONS
Our findings demonstrated clinical WUPyV strain was successfully isolated for the first time in the world and this will help unravel the etiology and pathogenic mechanisms of WUPyV in respiratory infection diseases.
背景
华盛顿大学多瘤病毒（WUPyV）是一种新型的人类多瘤病毒，于 2007 年在患有急性呼吸道感染的儿童中发现。然而，由于缺乏合适的体外培养系统，WUPyV 与呼吸道疾病的关系尚未确定。
方法
为了使用人呼吸道上皮（HAE）细胞分离 WUPyV，将阳性样本孵育在 HAE 中，然后分别使用实时 PCR、免疫荧光和电子显微镜检测核酸、VP1 蛋白和病毒粒子。
结果
结果表明，WUPyV 可以在 HAE 细胞中有效复制，可以观察到具有典型多瘤病毒特征的病毒粒子。此外，还获得了分离株（BJ0771）的全基因组序列，系统进化分析表明 BJ0771 属于基因簇 I。
结论
我们的研究结果首次证明了临床 WUPyV 株在世界范围内的成功分离，这将有助于揭示 WUPyV 在呼吸道感染疾病中的病因和发病机制。
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