Department of Anatomy, Tarbiat Modares University, PO Box 14115-111, Tehran, Iran.
Hum Reprod. 2010 Apr;25(4):977-85. doi: 10.1093/humrep/deq002. Epub 2010 Feb 6.
The aim of this study was to investigate the effect of sodium selenite (SS) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and glutathione peroxide (GPx) activity of cultured pre-antral follicles derived from vitrified and non-vitrified ovarian tissue.
Immature mouse ovaries were vitrified, and mechanically isolated pre-antral follicles from vitrified and non-vitrified samples were cultured in TCM 199 medium supplemented with different concentrations (0, 5 and 10 ng/ml) of SS. Follicular, oocyte and embryo development was assessed. In parallel, ROS, TAC and GPx levels were analyzed after 0, 12, 24, 48, 72 and 96 h of culture.
Development rates of follicles, oocytes and embryos were significantly higher in SS-supplemented groups (P < 0.005). ROS production was increased, and TAC levels and GPx activities were decreased after 24 h of culture of pre-antral follicles in vitrified and non-vitrified groups, whereas in the presence of SS, ROS production was decreased and TAC levels and selenium-dependent GPx-specific activities were increased after 96 h of culture. Vitrified and non-vitrified samples responded in a similar manner.
SS caused an increase in follicular TAC level and GPx activity and a decrease in ROS level, thus improving the in vitro development of follicles.
本研究旨在探讨亚硒酸钠（SS）对玻璃化和非玻璃化卵巢组织来源的原始卵泡中活性氧（ROS）产生、总抗氧化能力（TAC）和谷胱甘肽过氧化物酶（GPx）活性的影响。
对不成熟的小鼠卵巢进行玻璃化处理，并用机械方法从玻璃化和非玻璃化的样本中分离原始卵泡，并在添加不同浓度（0、5 和 10 ng/ml）SS 的 TCM 199 培养基中培养。评估卵泡、卵母细胞和胚胎的发育情况。同时，在培养 0、12、24、48、72 和 96 小时后分析 ROS、TAC 和 GPx 水平。
添加 SS 的组中卵泡、卵母细胞和胚胎的发育率显著提高（P < 0.005）。在玻璃化和非玻璃化组的原始卵泡培养 24 小时后，ROS 产生增加，TAC 水平和 GPx 活性降低，而在 SS 存在的情况下，ROS 产生减少，TAC 水平和硒依赖性 GPx 特异性活性增加培养 96 小时后。玻璃化和非玻璃化样本的反应方式相似。
SS 导致卵泡 TAC 水平和 GPx 活性增加，ROS 水平降低，从而改善卵泡的体外发育。
