Kobayashi Yuka, Sato Koichiro, Kibe Tatsuya, Seimiya Hiroyuki, Nakamura Asako, Yukawa Masashi, Tsuchiya Eiko, Ueno Masaru
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan.
Biosci Biotechnol Biochem. 2010;74(2):382-5. doi: 10.1271/bbb.90496. Epub 2010 Feb 7.
Replication protein A (RPA) binds to single-stranded DNA generated during DNA replication and other processes. The roles of RPA in telomere maintenance have been demonstrated in yeasts, but not in telomerase-positive human cells. In this study, we found that expression of mutant RPA70 in human cells caused telomere shortening, suggesting that RPA is required for telomere-length regulation in human cancer cells.
复制蛋白A（RPA）与DNA复制及其他过程中产生的单链DNA结合。RPA在端粒维持中的作用已在酵母中得到证实，但在端粒酶阳性的人类细胞中尚未得到证实。在本研究中，我们发现人类细胞中突变型RPA70的表达导致端粒缩短，这表明RPA是人类癌细胞中端粒长度调控所必需的。
