有证据表明，克氏锥虫动质体DNA微小环的全长都被转录。

Evidence that the entire length of a kinetoplast DNA minicircle is transcribed in Trypanosoma cruzi.

作者信息

Thertulien R, Harth G, Haidaris C G

机构信息

Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, New York 14642.

出版信息

Mol Microbiol. 1991 Jan;5(1):207-15. doi: 10.1111/j.1365-2958.1991.tb01841.x.

DOI:10.1111/j.1365-2958.1991.tb01841.x

PMID:2014000

Abstract

A 1.3 kb cDNA (cDNA52) was derived from Trypanosoma cruzi trypomastigote mRNA. Using single stranded probes in Northern blots, we identified the putative coding strand of cDNA52. In addition, a minor band was detected in RNA from epimastigotes that was absent in RNA from trypomastigotes. Nucleotide sequence analysis revealed that cDNA52 was highly homologous to T. cruzi kinetoplast DNA minicircle sequences. All four conserved regions of T. cruzi minicircles were identified in cDNA52. Using several criteria, we demonstrated that the hybridization signals were not caused by contaminating minicircle DNA in the RNA preparations. The data provide direct evidence for the unprecedented finding that the entire length of a kDNA minicircle is transcribed in T. cruzi.

摘要

一个1.3 kb的cDNA（cDNA52）源自克氏锥虫的锥鞭毛体mRNA。在Northern印迹中使用单链探针，我们鉴定出了cDNA52的推定编码链。此外，在无鞭毛体的RNA中检测到一条小条带，而在锥鞭毛体的RNA中不存在。核苷酸序列分析表明，cDNA52与克氏锥虫动质体DNA小环序列高度同源。在cDNA52中鉴定出了克氏锥虫小环的所有四个保守区域。使用多种标准，我们证明杂交信号不是由RNA制剂中污染的小环DNA引起的。这些数据为克氏锥虫中转录了整个动质体DNA小环这一前所未有的发现提供了直接证据。