Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, Tianjin, China.
Genome. 2010 Feb;53(2):119-24. doi: 10.1139/g09-092.
Thermosensitive genic male-sterile (TGMS) rice is widely used in hybrid rice production. Because of its specific temperature requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered TGMS line, G20S, has an opposite phenotype compared with normal TGMS lines. G20S is completely sterile when the temperature is lower than 29.5 degrees C. Thus, it can be widely used in a larger area. Genetic analysis indicated that the sterility of G20S was controlled by a single recessive gene that was named tms6(t). An F2 population from the cross between G20S and Jing226 was developed and used for molecular mapping of the tms6(t) gene. The simple sequence repeat technique, combined with bulked segregant analysis, was used to screen markers linked to the target gene, and linkage markers such as RM216, RM2504, RM3152, and RM4455 were identified. These markers are all located on chromosome 10; therefore, tms6(t) was subsequently mapped on chromosome 10. Co-segregation analysis using the F2 population showed that two of these markers, RM3152 and RM4455, were closely linked to tms6(t) at distances of 3.00 cM and 1.10 cM, respectively. Additionally, integration of the genetic and physical maps showed that there were 15 BAC/PAC clones overlapping between RM3152 and RM4455, and tms6(t) was mapped to an interval of 1455 kb.
温敏雄性不育（TGMS）水稻广泛应用于杂交水稻生产。由于其对温度的特殊要求，它只能在亚洲的一个狭窄的水稻种植区使用。新发现的 TGMS 系 G20S 与正常 TGMS 系的表型相反。当温度低于 29.5°C 时，G20S 完全不育。因此，它可以在更大的区域内广泛使用。遗传分析表明，G20S 的不育性由一个单隐性基因控制，该基因被命名为 tms6(t)。G20S 与 Jing226 杂交产生的 F2 群体被开发出来，并用于 tms6(t)基因的分子图谱绘制。简单序列重复技术与混池分离分析相结合，用于筛选与目标基因连锁的标记，鉴定到 RM216、RM2504、RM3152 和 RM4455 等连锁标记。这些标记都位于第 10 号染色体上；因此，tms6(t)随后被定位在第 10 号染色体上。利用 F2 群体进行共分离分析表明，这两个标记 RM3152 和 RM4455 与 tms6(t)紧密连锁，距离分别为 3.00 cM 和 1.10 cM。此外，遗传图谱和物理图谱的整合表明，在 RM3152 和 RM4455 之间有 15 个 BAC/PAC 克隆重叠，tms6(t)被定位在 1455 kb 的区间内。
