Zhang Xiao, Chang Guimei, Wu Zihao, Wan Jinpeng, Yang Jun, Wang Feijun, Wang Fang, Yu Diqiu, Xu Peng
CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Plant Divers. 2021 Jun 1;44(2):213-221. doi: 10.1016/j.pld.2021.05.002. eCollection 2022 Mar.
Thermosensitive genic male sterility (TGMS) has been widely used in two-line hybrid rice breeding. Due to hybrid seed production being highly affected by changeable environments, its application scope is limited to some extent. Thus, it is of great importance to identify potential TGMS genes in specific rice varieties. Here, Diannong S-1 xuan (DNS-1X), a reverse TGMS (RTGMS) male sterile line, was identified from Diannong S-1. Genetic analysis showed that male sterility was tightly controlled by a single recessive gene, which was supported by the phenotype of the F and F populations derived from the cross between DNS-1X and Yunjing 26 (YJ26). Combining simple sequence repeat (SSR) markers and bulked segregation analysis (BSA), we identified a 215 kb region on chromosome 10 as a candidate reverse TGMS region, which was designated as . It was narrower than the previously reported RTGMS genes . The fertility conversion detected in the natural environment showed that DNS-1X was sterile below 28-30 °C; otherwise, it was fertile. Histological analysis further indicated that the pollen abortion was occurred in the young microspore stage. This study will provide new resources for two-line hybrid rice and pave the way for molecular breeding of RTGMS lines.
温敏雄性不育（TGMS）已广泛应用于两系杂交水稻育种。由于杂交种子生产受多变环境的影响很大，其应用范围在一定程度上受到限制。因此，在特定水稻品种中鉴定潜在的TGMS基因具有重要意义。在此，从滇农S-1中鉴定出一个反向温敏雄性不育（RTGMS）雄性不育系滇农S-1选（DNS-1X）。遗传分析表明，雄性不育受单个隐性基因严格控制，这一点得到了DNS-1X与云粳26（YJ26）杂交所得F和F群体表型的支持。结合简单序列重复（SSR）标记和混合分组分析法（BSA），我们在第10号染色体上鉴定出一个215 kb的区域作为候选反向温敏雄性不育区域，命名为。它比先前报道的RTGMS基因区域更窄。在自然环境中检测到的育性转换表明，DNS-1X在28-30℃以下不育；否则，它是可育的。组织学分析进一步表明，花粉败育发生在幼嫩小孢子阶段。本研究将为两系杂交水稻提供新的资源，并为RTGMS系的分子育种铺平道路。
