[智利圣地亚哥一家公立医院和私人诊所子宫颈癌患者的人乳头瘤病毒（HPV）基因型]

[Human papillomavirus (HPV) genotypes in cervix uterine cancer patients in a public hospital and private clinic from Santiago, Chile].

作者信息

Valdivia L Isabel M, Aguayo G Francisco, Pruyas A Martha, Snijders Peter J F, Corvalán R Alejandro, Ferreccio R Catterina

机构信息

Laboratorio de Patología Molecular y Epidemiología, Centro de Investigaciones Médicas, Pontificia Universidad Católica de Chile, Santiago, Chile.

出版信息

Rev Chilena Infectol. 2010 Feb;27(1):11-6. Epub 2010 Feb 3.

PMID:20140308

Abstract

We compared HPV genotypes among squamous cervical cancer samples from a public hospital (n = 55) and a private clinic (n = 35 cases) of Santiago. Paraffin-embedded specimens were analyzed by PCR followed by an immunoenzimatic assay. Reverse line blotting was used for the identification of 36 HPV genotypes. We found HPVDNAm 94.4% of all cancers. Single mfections: HPV16: 40.0%, (clinic 37.1%, hospital 41.8%) VPH18:7.8% (clinic 2.9%, hospital 10.9%); single+multiple mfections: VPH16: 61.1% (clinic 53.1%, hospital 71.7%), VPH18: 34.4% (clinic 21.9%, hospital 45.2%). HPV16 orHPV18 occurredin 75.6% of cases, higher in the hospital than the clinic (87.3%-95% CI: 84.9-96.3 - and 57. l%-95% CI: 46.6-66 - respectively, p = 0.002). Other genotypes in single mfections: HPV 26, 31, 33, 45, 58, 67; in co-mfections: HPV 35,52,56,59 and 66. HPV16 but specially HPV 18 were significantly more frequent in the public hospital; 75.6% of squamous cervical cancer were associated to the vaccine preventable HPV16/18.

摘要

我们比较了圣地亚哥一家公立医院（n = 55）和一家私人诊所（n = 35例）的宫颈鳞状癌样本中的HPV基因型。通过PCR随后进行免疫酶测定分析石蜡包埋标本。采用反向线印迹法鉴定36种HPV基因型。我们在所有癌症中发现HPV DNA的比例为94.4%。单一感染：HPV16：40.0%，（诊所37.1%，医院41.8%）；HPV18：7.8%（诊所2.9%，医院10.9%）；单一感染+多重感染：HPV16：61.1%（诊所53.1%，医院71.7%），HPV18：34.4%（诊所21.9%，医院45.2%）。75.6%的病例中出现HPV16或HPV18，在医院中的比例高于诊所（分别为87.3%-95%CI：84.9-96.3和57.1%-95%CI：46.6-66，p = 0.002）。单一感染中的其他基因型：HPV 26、31、33、45、58、67；合并感染中的基因型：HPV 35、52、56、59和66。HPV16尤其是HPV 18在公立医院中明显更常见；75.6%的宫颈鳞状癌与疫苗可预防的HPV16/18相关。