Department of Medical Biology, Faculty of Medicine, Harran University, Sanliurfa, Turkey.
Parasitol Res. 2010 Mar;106(4):783-8. doi: 10.1007/s00436-009-1710-8. Epub 2010 Feb 6.
Malaria is a complex disease that varies widely in epidemiology and clinical manifestation in the southeastern part of Turkey. In many regions of the world, chloroquine (CQ) has been the standard treatment for Plasmodium vivax. However, the resistance of the Plasmodium species to antimalarial drugs has become an increasing problem and a concern worldwide. Our target was to determine the Plasmodium species in the southeast region of Turkey and the therapeutic efficacy of CQ used in the treatment of malaria. Blood samples were collected from 180 patients infected with malaria before and after CQ treatment and were subjected to DNA isolation. The isolated DNA was amplified by a seminested multiplex polymerase chain reaction (SnM-PCR) including primers selected on Plasmodium small subunit ribosomal RNA (ssrRNA) genes for identification of the malaria species. The SnM-PCR results showed that only P. vivax exists in this province. It was also determined that there is a therapeutic failure to CQ in 9.5% of patients. These were the second report on identification of P. vivax and the third report on determination of the therapeutic failure in patients who used CQ to cure human malaria in the southeastern region of Turkey. Our results demonstrate that the SnM-PCR is a sensitive, specific, and a rapid tool for the differentiation of malaria species.
疟疾是一种复杂的疾病，在土耳其东南部地区，其流行病学和临床表现差异很大。在世界许多地区，氯喹（CQ）一直是治疗间日疟原虫的标准治疗方法。然而，疟原虫对抗疟药物的耐药性已成为全球日益关注的问题。我们的目标是确定土耳其东南部地区的疟原虫种类以及 CQ 治疗疟疾的疗效。采集了 180 名感染疟疾患者在 CQ 治疗前后的血样，并进行 DNA 分离。从 Plasmodium small subunit ribosomal RNA (ssrRNA) 基因中选择引物，对分离的 DNA 进行半巢式多重聚合酶链反应（SnM-PCR）扩增，以鉴定疟原虫种类。SnM-PCR 结果表明，该省仅存在间日疟原虫。还确定在 9.5%的患者中对 CQ 治疗存在治疗失败。这是土耳其东南部地区第二次报告鉴定间日疟原虫，也是第三次报告使用 CQ 治愈人类疟疾的患者治疗失败的报告。我们的结果表明，SnM-PCR 是一种敏感、特异、快速的疟疾物种鉴定工具。
