Wang Jian, Zhao Fuxi, Wu Yongming, Liu Runhua, Mu Yaqin
Department of Immunology, Shanxi Medical University, Taiyuan, Shanxi, 030001 P.R.China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Feb;27(1):73-6. doi: 10.3760/cma.j.issn.1003-9406.2010.01.016.
To investigate the level of hypermethylated ras association domain family 1A (RASSF1A) gene in maternal plasma of pre-eclampsia and its clinical value.
Sixty pre-eclampsia women including 30 mild and 30 severe cases were selected, 60 women with normal pregnancy were studied as control. Free DNA from plasma samples was extracted, fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect the concentrations of RASSF1A gene before and after methylation-sensitive restriction digestion. Meanwhile, beta-actin gene was detected as a control to confirm complete enzyme digestion.
The median concentration of hypermethylated RASSF1A gene was 3.31-fold higher in samples from pre-eclamptic pregnancies than that in controls. There was significant difference between the mild and severe pre-eclamptic subjects (P<0.05), with the median concentrations of 1659 copies/mL and 2036.50 copies/mL, respectively.
Hypermethylated RASSF1A gene in pre-eclampsia plasma was significantly increased and the concentrations were related to the severity of pre-eclampsia.
探讨子痫前期孕妇血浆中RAS相关结构域家族1A（RASSF1A）基因的甲基化水平及其临床价值。
选取60例子痫前期孕妇，其中轻度30例，重度30例，选取60例正常孕妇作为对照。提取血浆样本中的游离DNA，采用荧光定量聚合酶链反应（FQ-PCR）检测甲基化敏感限制性消化前后RASSF1A基因的浓度。同时，检测β-肌动蛋白基因作为对照，以确认酶切完全。
子痫前期孕妇样本中甲基化RASSF1A基因的中位浓度比对照组高3.31倍。轻度和重度子痫前期患者之间存在显著差异（P<0.05），中位浓度分别为1659拷贝/毫升和2036.50拷贝/毫升。
子痫前期血浆中甲基化RASSF1A基因显著增加，且浓度与子痫前期的严重程度相关。
