Tsui Dana W Y, Chan K C Allen, Chim Stephen S C, Chan Lin-Wai, Leung Tak-Yeung, Lau Tze-Kin, Lo Y M Dennis, Chiu Rossa W K
Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR.
Prenat Diagn. 2007 Dec;27(13):1212-8. doi: 10.1002/pd.1897.
To study if quantitative aberrations in circulating placental-derived hypermethylated RASSF1A DNA in maternal plasma are associated with pre-eclamptic pregnancies.
Maternal plasma and placental tissues from third-trimester pre-eclamptic women and gestational-age matched normotensive controls were studied. Real-time PCR was performed to quantify RASSF1A concentrations before and after methylation-sensitive restriction digestion in a duplex assay, where ss-actin concentrations were quantified as an internal control to confirm complete enzyme digestion.
The median concentrations of hypermethylated RASSF1A were 4.3-fold higher in maternal plasma of pre-eclamptic subjects than in controls. There was no significant difference between the extent of RASSF1A hypermethylation in placental tissues obtained from pre-eclamptic and control pregnancies.
This study demonstrated the potential utility of hypermethylated RASSF1A sequences in maternal plasma as a gender- and polymorphism-independent marker for pre-eclampsia.
研究母体血浆中循环胎盘来源的高甲基化RASSF1A DNA的定量异常是否与子痫前期妊娠相关。
研究了孕晚期子痫前期妇女和孕周匹配的血压正常对照组的母体血浆和胎盘组织。采用双链分析法，在甲基化敏感限制性消化前后进行实时PCR定量RASSF1A浓度，其中ss-肌动蛋白浓度作为内对照进行定量，以确认酶切完全。
子痫前期患者母体血浆中高甲基化RASSF1A的中位浓度比对照组高4.3倍。子痫前期妊娠和对照妊娠胎盘组织中RASSF1A高甲基化程度无显著差异。
本研究证明母体血浆中高甲基化RASSF1A序列作为子痫前期的性别和多态性无关标志物具有潜在应用价值。
