Anal Chem. 2010 Mar 1;82(5):1621-3. doi: 10.1021/ac100008n.
Monolithic poly(styrene-co-vinylbenzyl chloride-co-divinylbenzene) precursor capillary columns have been prepared and then hypercrosslinked to afford a monolith containing an array of small pores. This monolithic column exhibited a surface area of 663 m(2)/g or more than 1 order of magnitude larger than measured for the precursor column. Because it contains mesopores, this monolithic column affords good separation of uracil and alkylbenzenes in isocratic mobile phase mode and also proved useful for separations in size exclusion mode. A column efficiency as high as 73,000 plates/m was determined for uracil. In contrast, the presence of mesopores in this hypercrosslinked monolithic column had a detrimental effect on the separation of proteins.
整体式多(苯乙烯-共-乙烯基苄基氯-共-二乙烯基苯)先驱毛细管柱已经被制备出来,然后超交联以提供含有一系列小孔的整体式柱。该整体式柱表现出 663 m²/g 或更大的表面积,比先驱柱的表面积大一个数量级以上。由于它含有中孔,这种整体式柱在等度流动相模式下能够很好地分离尿嘧啶和烷基苯,并且在尺寸排阻模式下的分离也很有用。对于尿嘧啶,测定的柱效率高达 73000 板/m。相比之下,中孔的存在在这种超交联整体式柱中对蛋白质的分离有不利影响。