Choudhury Sidratul, Fitzhenry Laurence, White Blánaid, Connolly Damian
National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
Pharmaceutical and Molecular Biotechnology Research Centre (PMBRC), Department of Science, Waterford Institute of Technology, Waterford, Ireland.
Materials (Basel). 2016 Mar 18;9(3):212. doi: 10.3390/ma9030212.
The reversed phase liquid chromatographic (RP-HPLC) separation of small molecules using a polystyrene--divinylbenzene (PSDVB) polyHIPE stationary phases housed within 1.0 mm i.d. silcosteel columns is presented within this study. A 90% PSDVB polyHIPE was covalently attached to the walls of the column housing by prior wall modification with 3-(trimethoxysilyl) propyl methacrylate and could withstand operating backpressures in excess of 200 bar at a flow rate of 1.2 mL/min. Permeability studies revealed that the monolith swelled slightly in 100% acetonitrile relative to 100% water but could nevertheless be used to separate five alkylbenzenes using a flow rate of 40 µL/min (linear velocity: 0.57 mm/s). Remarkable column-to-column reproducibility is shown with retention factor variation between 2.6% and 6.1% for two separately prepared columns.
本研究介绍了使用内径为1.0 mm的硅钢柱内填充的聚苯乙烯-二乙烯基苯（PSDVB）聚HIPE固定相进行小分子的反相液相色谱（RP-HPLC）分离。通过用甲基丙烯酸3-（三甲氧基甲硅烷基）丙酯预先对柱壳壁进行改性，将90%的PSDVB聚HIPE共价连接到柱壳壁上，并且在流速为1.2 mL/min时能够承受超过200 bar的操作背压。渗透性研究表明，相对于100%水，整体柱在100%乙腈中略有溶胀，但仍可用于以40 μL/min的流速（线速度：0.57 mm/s）分离五种烷基苯。两根单独制备的柱子的保留因子变化在2.6%至6.1%之间，显示出显著的柱间重现性。
