Institute of Hadong Green Tea, Hadong, Gyeongnam, 667-804, South Korea, Division of Forest Research, Gyeongsangnam-do Forest Environment Research Institute, Jinju, Gyeongnam, 660-870, South Korea.
J Nat Prod. 2010 Feb 26;73(2):147-50. doi: 10.1021/np900525n.
The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of 1 was enhanced in calli undergoing differentiation to shoot after 60 days of culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6beta-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids, was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli.
对莨菪属小花愈伤组织的分化和去分化过程中托烷生物碱的生成进行了研究。愈伤组织去分化过程中托烷生物碱的生成急剧减少。在培养 10 天后，莨菪碱（1）的生成减少，而在根到愈伤组织的去分化过程中，东莨菪碱（2）的生成减少。培养 60 天后，愈伤组织向芽分化，1 的生成增加，80 天达到最大值。然而，再生植物中莨菪碱的生成量较低。托烷生物碱生物合成的关键酶——东莨菪碱 6β-羟化酶（H6H）的表达水平在 4 周龄的愈伤组织中显著增加。本研究表明，托烷生物碱的生物合成在去分化的莨菪属小花愈伤组织中受到负调控。
