Key Laboratory of Macromolecular Synthesis and Functionalization (Ministry of Education), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Langmuir. 2010 Jun 1;26(11):8946-52. doi: 10.1021/la904729b.
Carbohydrate-protein interactions are critical in many biological processes. However, the interactions between individual carbohydrates and proteins are often of low affinity and difficult to study. Recent development of carbohydrate microarrays provides an effective tool to explore the interaction. In this work, carbohydrate microarrays were controllably constructed by grafting of a carbohydrate-containing monomer on self-organized honeycomb-patterned films. The films were prepared from an amphiphilic block copolymer, poly(styrene-block-(2-hydroxyethyl methacrylate)), by a breath figure method. Three-dimensional fluorescence results demonstrate that the hydroxyl groups aggregate mainly inside the pores, which afford a chance of site-directed surface modification. 2-(2,3,4,6-Tetra-O-acetyl-beta-D-glucosyloxy)ethyl methacrylate was selectively grafted in the pores by a surface-initiated atom transfer radical polymerization. Characterization by attenuated total reflectance Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy, and contact angle measurements confirms the site-directed growth of the glycopolymer chains. Further specific recognition of the carbohydrate microarrays to lectin (concanavalin A) leads to an organized microarray of protein, and hence this approach also opens a new route to fabricating other functional microarrays such as protein-patterned surfaces.
碳水化合物-蛋白质相互作用在许多生物过程中至关重要。然而，单个碳水化合物和蛋白质之间的相互作用通常亲和力较低，难以研究。碳水化合物微阵列的最新发展为探索相互作用提供了一种有效的工具。在这项工作中，通过将含碳水化合物的单体接枝到自组织的蜂窝状图案薄膜上来可控地构建碳水化合物微阵列。薄膜由两亲性嵌段共聚物聚（苯乙烯嵌段-（2-羟乙基甲基丙烯酸酯））通过呼吸图法制备。三维荧光结果表明，羟基主要在孔内聚集，这为定向表面修饰提供了机会。通过表面引发原子转移自由基聚合，在孔中选择性地接枝 2-（2,3,4,6-四-O-乙酰基-β-D-葡萄糖氧基）乙基甲基丙烯酸酯。衰减全反射傅里叶变换红外光谱、X 射线光电子能谱、扫描电子显微镜、原子力显微镜和接触角测量的表征证实了糖聚合物链的定向生长。碳水化合物微阵列对凝集素（刀豆球蛋白 A）的进一步特异性识别导致蛋白质的有序微阵列，因此这种方法也为制造其他功能微阵列（如蛋白质图案表面）开辟了新途径。
