Laboratoire de Chimie des Polymères Organiques, CNRS, Université Bordeaux I, ENSCPB. 16, Avenue Pey Berland 33607, Pessac-Cedex, France.
Langmuir. 2010 Jun 1;26(11):8552-8. doi: 10.1021/la904565d.
We prepared breath figure patterns on functional surfaces by the surface segregation of a statistical glycopolymer, (styrene-co-2-(D-glucopyranosyl) aminocarbonyloxy ethyl acrylate (S-HEAGl). The synthesis of the statistical glycopolymer is prepared in a straightforward approach by conventional free radical copolymerization of styrene and the unprotected glycomonomer. Blends of this copolymer and high-molecular-weight polystyrene were spin coated from THF solutions, leading to the formation of surfaces with both controlled functionality and topography. AFM studies revealed that both the composition of the blend and the relative humidity play key roles in the size and distribution of the pores at the interface. Thus, the topographical features obtained on the polymer surfaces during film preparation by the breath figure methodology varied from 200 to 700 nm. Moreover, this approach leads to porous films in which the hydrophilic glycomonomer units are oriented toward the pore interface because upon soft annealing in water the holes are partially swelled. The self-organization of the glycopolymer within the pores was additionally confirmed by the reaction of carbohydrate hydroxyl groups with rhodamine isocyanate. Equally, we demonstrate the bioactivity of the anchored glycopolymers by means of the lectin binding test using concanavalin A (Con A).
我们通过统计糖基聚合物（苯乙烯-co-2-（D-葡萄糖基）氨甲酰氧基乙基丙烯酰胺（S-HEAGl）的表面分相，在功能表面上制备了呼吸图案。统计糖基聚合物的合成是通过苯乙烯和未保护的糖单体的常规自由基共聚以直接的方法制备的。将该共聚物和高分子量聚苯乙烯的混合物从 THF 溶液中旋涂，导致具有受控功能和形貌的表面的形成。AFM 研究表明，共混物的组成和相对湿度在界面处的孔的大小和分布中起着关键作用。因此，通过呼吸图形方法在膜制备过程中在聚合物表面上获得的形貌特征从 200nm 到 700nm 变化。此外，由于在水中进行软退火时孔部分溶胀，这种方法导致了多孔膜，其中亲水糖单体单元朝向孔界面取向。糖聚合物在孔内的自组织还通过与罗丹明异氰酸酯的碳水化合物羟基的反应得到证实。同样，我们通过使用伴刀豆球蛋白 A（Con A）的凝集素结合试验证明了固定化糖聚合物的生物活性。
