Fiber and Polymer Science Program, Department of Fiber Science & Apparel Design, Cornell University, Ithaca, New York 14853-4401, USA.
Langmuir. 2010 Feb 16;26(4):2831-8. doi: 10.1021/la902855e.
Amine groups are the building units of proteins. The incorporation of amine groups into polyethylene glycol diacrylate (PEGDA) hydrogel through dextran-allyl isocyanate-ethylamine (Dex-AE) enhances sustained protein release by introducing effective interactions. To investigate such an interaction effect and to improve protein release, we impregnated self-assembled tubular structures from dextran-bromoethylamine (Dex-BH) and dextran-chloroacetic acid (Dex-CA) into Dex-AE/PEGDA hydrogel. The morphology data obtained from scanning electron microscopy (SEM) reveal that pure PEGDA hydrogel had no effect on the distribution of the self-assembled tubules; the introduction of Dex-AE brought about the dispersion of these tubules, and an increase in Dex-AE content led to more evenly distributed structures. Moreover, the implantation of the self-assembled tubules had no distinct effect on the swelling capacity of the hybrid self-assembly embedded hydrogels. The in vitro albumin release study was carried out in a pH 7.4 buffer solution; the results show that the implantation of the self-assembly into the hydrogels reduced the burst release and prolonged the protein release time. These findings demonstrate that the impregnation of tubular self-assembly into hydrogel makes the hybrid hydrogel an excellent protein delivery system.
胺基是蛋白质的组成单元。通过葡聚糖-丙烯酰异氰酸酯-乙胺（Dex-AE）将胺基引入聚乙二醇二丙烯酸酯（PEGDA）水凝胶中，通过引入有效相互作用来增强蛋白质的持续释放。为了研究这种相互作用效应并改善蛋白质的释放，我们将葡聚糖-溴乙胺（Dex-BH）和葡聚糖-氯乙酸（Dex-CA）自组装管状结构浸渍到 Dex-AE/PEGDA 水凝胶中。扫描电子显微镜（SEM）获得的形态数据表明，纯 PEGDA 水凝胶对自组装管的分布没有影响；Dex-AE 的引入导致这些管的分散，并且 Dex-AE 含量的增加导致结构分布更加均匀。此外，自组装管的植入对混合自组装嵌入水凝胶的溶胀能力没有明显影响。在 pH7.4 的缓冲溶液中进行了体外白蛋白释放研究；结果表明，将自组装体植入水凝胶中减少了突释，并延长了蛋白质的释放时间。这些发现表明，将管状自组装体浸渍到水凝胶中使混合水凝胶成为一种优异的蛋白质递送系统。
