Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, National Chiao Tung University, Hsinchu, Taiwan.
Med Mycol. 2010 Mar;48(2):328-34. doi: 10.3109/13693780903154070.
To study the demographic changes of yeasts causing invasive infections in Taiwan, especially with respect to species distribution and antifungal susceptibility, we analyzed isolates obtained from four sterile sites of patients in 19 hospitals in 2002 (155 strains) and again from the same hospitals in 2006 (208 strains). Blood was the most common source of the yeasts, accounting for 73.8% of the total isolates, followed by ascites (21.5%), cerebrospinal fluid (3%), and synovia (1.7%). Candida albicans was the most frequently recovered species (50.1% of the total), followed by Candida tropicalis (20.7%), Candida glabrata (11.6%), Candida parapsilosis (8.5%), Cryptococcus neoformans (3.9%), Candida krusei (0.8%), and nine other species (4.3%). There were one (0.3%) and seven (1.9%) isolates with minimum inhibitory concentrations (MICs) of amphotericin B > or =2 mg/l after 24 h and 48 h incubation, respectively. In addition, there were 15 (4.3%) and 31 (8.6%) isolates with MICs of fluconazole > or =64 mg/l under the same conditions. The MIC(90) value of amphotericin B was 1 mg/l. The MIC(90) values of fluconazole were 4 mg/l after 24 h incubation and 32 mg/l after 48 h incubation. Interestingly, MICs for fluconazole > or =64 mg/l after 24 h were significantly higher for isolates obtained in 2006 than those in 2002 after 24 h (7.1% vs. 0.7%, p =0.009) and 48 h (13.5% vs. 2%, p =0.0003) incubations. The demographic difference between these two surveys is mainly due to one species, C. tropicalis.
为了研究引起台湾侵袭性真菌感染的酵母菌的人口统计学变化，特别是关于种属分布和抗真菌药物敏感性，我们分析了 2002 年从 19 家医院的 4 个无菌部位的患者分离的菌株（155 株），并于 2006 年再次从同一家医院分离（208 株）。血液是酵母菌最常见的来源，占总分离株的 73.8%，其次是腹水（21.5%）、脑脊液（3%）和滑液（1.7%）。白念珠菌是最常被回收的菌种（占总回收菌的 50.1%），其次是热带念珠菌（20.7%）、光滑念珠菌（11.6%）、近平滑念珠菌（8.5%）、新型隐球菌（3.9%）、克柔念珠菌（0.8%）和其他 9 种（4.3%）。有 1 株（0.3%）和 7 株（1.9%）在 24 小时和 48 小时孵育后最低抑菌浓度（MIC）大于或等于两性霉素 B 2mg/L。此外，在相同条件下，有 15 株（4.3%）和 31 株（8.6%）的氟康唑 MIC 大于或等于 64mg/L。两性霉素 B 的 MIC（90）值为 1mg/L。氟康唑 24 小时孵育后 MIC（90）值为 4mg/L，48 小时孵育后 MIC（90）值为 32mg/L。有趣的是，在 24 小时孵育时，氟康唑 MIC 大于或等于 64mg/L 的菌株在 2006 年的分离率明显高于 2002 年，24 小时时分别为 7.1%和 0.7%（p=0.009），48 小时时分别为 13.5%和 2%（p=0.0003）。这两次调查之间的人口统计学差异主要是由于一种物种，热带念珠菌。
