Shimozono Naoki, Ohta Naoyuki, Satoh Nori, Hamada Mayuko
Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Zoolog Sci. 2010 Feb;27(2):103-9. doi: 10.2108/zsj.27.103.
Our previous transcriptome analysis identified 565 genes that are preferentially expressed in the developing brain of Ciona intestinalis larvae. Here, we show by in-situ hybridization that the spatial expression patterns of these brain-specific genes fall into different categories depending on the regions where the gene is expressed. For example, Ci-opsin3 and Ci-Dkk3 are expressed in the entire brain, Ci-tyrosinase and Ci-TYRP1 in the dorsal region, and Ci-synaptotagmin3, Ci-ZF399, and Ci-PTFb in the ventral region. Other genes are specific to the posterior, anterior, central, posterior and ventral, or anterior-ventral region of the brain. This regional expression of genes in the Ciona brain is not always associated with cell lineage, suggesting that complex mechanisms control the regionalized expression of brain-specific genes.
我们之前的转录组分析确定了565个在玻璃海鞘幼虫发育中的大脑中优先表达的基因。在此，我们通过原位杂交表明，这些大脑特异性基因的空间表达模式根据基因表达的区域分为不同类别。例如，Ci-视蛋白3和Ci-Dkk3在整个大脑中表达，Ci-酪氨酸酶和Ci-TYRP1在背侧区域表达，Ci-突触结合蛋白3、Ci-ZF399和Ci-PTFb在腹侧区域表达。其他基因则特异性表达于大脑的后部、前部、中部、后部和腹侧或前腹侧区域。玻璃海鞘大脑中基因的这种区域表达并不总是与细胞谱系相关，这表明复杂的机制控制着大脑特异性基因的区域化表达。
