Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
Lett Appl Microbiol. 2010 May;50(5):537-41. doi: 10.1111/j.1472-765X.2010.02807.x. Epub 2010 Jan 22.
To analyse the occurrence of faecal carriage of vancomycin-resistant enterococci (VRE) in Buteo buteo and to study the associated resistance and virulence genes.
The presence of VRE was investigated in 33 faecal samples of B. buteo. Samples were seeded in Slanetz-Bartley agar plates supplemented with vancomycin for VRE recovery. Genes encoding antimicrobial resistance and virulence were studied by polymerase chain reaction. Vancomycin-resistant Enterococcus faecium isolates were characterized by multilocus sequence typing. VRE with an acquired mechanism of resistance (vanA genotype) were detected in 9% of samples analysed (Ent. faecium and Enterococcus durans). In addition, 27% of samples contained VRE with an intrinsic mechanism of resistance (Enterococcus gallinarum, vanC1). All vanA-containing isolates showed resistance to tetracycline and erythromycin and harboured the tet(M) and/or tet(L) genes, in addition to the ermB gene. The vat(E) and/or vat(D), cat(A) and aph(3')-IIIa genes were identified in quinupristin-dalfopristin-, chloramphenicol-, and kanamycin-resistant vanA-containing strains, respectively. The sequence types ST273 and ST5 were identified in two vanA-positive Ent. faecium isolates, and the presence of hyl, gelE, cylA, cylL and cylM virulence genes and gelatinase activity were identified in Ent. faecium ST5 strain.
The intestinal tract of B. buteo could be a reservoir of vanA-positive enterococci.
First study focused to define the occurrence of vanA-containing Enterococcus strains in B. buteo.
分析普通鵟肠道中产万古霉素耐药肠球菌（VRE）的携带情况，并研究相关耐药和毒力基因。
对 33 份普通鵟粪便样本进行了 VRE 检测。将样本接种于添加万古霉素的 Slanetz-Bartley 琼脂平板上，以回收 VRE。采用聚合酶链反应（PCR）研究了编码抗菌药物耐药和毒力的基因。通过多位点序列分型（MLST）对万古霉素耐药肠球菌分离株进行了表征。在 9%的分析样本中检测到获得性耐药机制（vanA 基因型）的 VRE（屎肠球菌和 Durans 肠球菌）。此外，27%的样本含有固有耐药机制（肠球菌鸡肠球菌，vanC1）的 VRE。所有携带 vanA 的分离株均对四环素和红霉素耐药，并携带 tet(M)和/或 tet(L)基因，此外还有 ermB 基因。在对奎奴普丁/达福普汀、氯霉素和卡那霉素耐药的携带 vanA 的菌株中，分别鉴定出 vat(E)和/或 vat(D)、cat(A)和 aph(3')-IIIa 基因。在两个携带 vanA 的屎肠球菌分离株中鉴定出 ST273 和 ST5 型，在屎肠球菌 ST5 株中鉴定出 hyl、gelE、cylA、cylL 和 cylM 毒力基因和明胶酶活性。
普通鵟的肠道可能是携带 vanA 的肠球菌的储库。
首次研究聚焦于确定普通鵟中携带 vanA 的肠球菌菌株的发生情况。
