Centre for Genomics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
Vet J. 2012 Jul;193(1):301-3. doi: 10.1016/j.tvjl.2011.12.007. Epub 2012 Jan 20.
The prevalence of vancomycin resistant-enterococci (VRE) in faecal samples from cattle, sheep and pigs slaughtered for human consumption was evaluated. Enterococci containing the vanA gene were detected in 25.3% and 2.7% of the porcine and ovine samples, respectively, and were identified as Enterococcus faecium. No vanA-containing enterococcal strains were detected in bovine samples. Enterococcal strains with intrinsic vancomycin resistance were detected in seven (9.9%) faecal samples from pigs and in two samples from both cattle and sheep (3.7% and 2.7%, respectively). All vanA-positive isolates from pigs were resistant to tetracycline and erythromycin, and the mobile element Tn916/Tn1545-like transposon was detected in 90.5% of the tetracycline-resistant isolates that contained the tet(M) gene. Although gelatinase and haemolytic activity were not detected, the hyl and cylB virulence genes were found within the VRE strains isolated.
评估了用于人类消费的屠宰牛、羊和猪的粪便样本中耐万古霉素肠球菌（VRE）的流行情况。在猪和羊的样本中分别检测到 25.3%和 2.7%的含有 vanA 基因的肠球菌，且被鉴定为屎肠球菌。在牛的样本中未检测到含 vanA 的肠球菌菌株。在来自猪的 7 份（9.9%）粪便样本和来自牛和羊的各 2 份样本（3.7%和 2.7%）中检测到具有固有万古霉素耐药性的肠球菌菌株。所有来自猪的 vanA 阳性分离株均对四环素和红霉素耐药，并且在含有 tet(M) 基因的 90.5%的四环素耐药分离株中检测到 Tn916/Tn1545 样转座子。尽管未检测到明胶酶和溶血活性，但在分离的 VRE 菌株中发现了 hyl 和 cylB 毒力基因。
