Mei Wu-xuan, Lao Shao-xian, Yu Na, Zhou Zheng, Huang Lie-ping, Hu Bin
Department of Internal Medicine, Xianning Medical College, Xianning 437100, Hubei Province, China.
Zhong Xi Yi Jie He Xue Bao. 2010 Feb;8(2):111-5. doi: 10.3736/jcim20100203.
To explore the relationship between gene expressions of aquaporin (AQP) 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis (CSG).
Twenty-four CSG patients were divided into three groups according to the degrees of spleen-stomach dampness-heat syndrome: mild, moderate and severe groups. There were 8 patients in each group, and another 8 healthy persons were selected as normal control. Samples of mucosa of upper stomach in the included patients and normal persons were collected by gastroscopy, and then were stored in liquid nitrogen. The gene expressions of AQP3 and AQP4 in gastric tissue were examined by fluorescence quantitative polymerase chain reaction.
The gene expressions of AQP3 and AQP4 in the moderate and severe groups were higher than that in the mild group and normal group (P<0.05, P<0.01). The gene expression of AQP3 in the severe group was higher than that in the moderate group (P<0.05).
The gene expressions of AQP3 and AQP4 in gastric mucosa are different in patients with various degrees of spleen-stomach dampness-heat syndrome of CSG. There is a relationship between expressions of AQP3 and AQP4 genes and the degrees of spleen-stomach dampness-heat syndrome, and the gene expressions will increase with the aggravation of the dampness-heat syndrome.
探讨水通道蛋白（AQP）3和4基因表达与慢性浅表性胃炎（CSG）脾胃湿热证不同程度之间的关系。
将24例CSG患者按脾胃湿热证程度分为三组：轻度、中度和重度组。每组8例患者，另选8例健康人作为正常对照。通过胃镜收集纳入患者及正常人的胃上部黏膜样本，然后储存在液氮中。采用荧光定量聚合酶链反应检测胃组织中AQP3和AQP4的基因表达。
中度和重度组AQP3和AQP4的基因表达高于轻度组和正常组（P<0.05，P<0.01）。重度组AQP3的基因表达高于中度组（P<0.05）。
CSG不同程度脾胃湿热证患者胃黏膜中AQP3和AQP4的基因表达存在差异。AQP3和AQP4基因表达与脾胃湿热证程度之间存在关联，且基因表达随湿热证加重而升高。
