大泡性角膜病变角膜中转化生长因子-β亚型的差异表达
Differential expression of transforming growth factor-beta isoforms in bullous keratopathy corneas.
作者信息
Strzalka-Mrozik Barbara, Stanik-Walentek Agnieszka, Kapral Malgorzata, Kowalczyk Malgorzata, Adamska Jolanta, Gola Joanna, Mazurek Urszula
机构信息
Department of Molecular Biology, Medical University of Silesia, Sosnowiec, Poland.
出版信息
Mol Vis. 2010 Feb 5;16:161-6.
PURPOSE
The aim of this study was to investigate transcriptional activities of genes encoding transforming growth factor (TGF)-beta isoforms in bullous keratopathy corneas.
METHODS
The study group consisted of 45 patients with bullous keratopathy (22 females and 23 males). The control group included 45 corneal donors (21 females and 24 males). Quantification of TGF-beta1, TGF-beta2, and TGF-beta3 mRNAs was performed by real-time quantitative reverse transcription PCR (QRT-PCR).
RESULTS
TGF-beta1, TGF-beta2, and TGF-beta3 mRNAs were detected in both normal and pseudophakic bullous keratopathy (PBK) corneas. We found significantly lower transcriptional activity of TGF-beta3 mRNA in bullous keratopathy corneas compared to normal tissues. TGF-beta1 and TGF-beta2 expressions were at the same level in both PBK and healthy corneas.
CONCLUSIONS
Downregulation of TGF-beta3 gene expression may play a significant role in molecular changes observed in bullous keratopathy.
目的
本研究旨在调查大泡性角膜病变角膜中编码转化生长因子（TGF）-β亚型的基因的转录活性。
方法
研究组由45例大泡性角膜病变患者（22例女性和23例男性）组成。对照组包括45例角膜供体（21例女性和24例男性）。通过实时定量逆转录PCR（QRT-PCR）对TGF-β1、TGF-β2和TGF-β3 mRNA进行定量分析。
结果
在正常角膜和人工晶状体植入后大泡性角膜病变（PBK）角膜中均检测到TGF-β1、TGF-β2和TGF-β3 mRNA。我们发现，与正常组织相比，大泡性角膜病变角膜中TGF-β3 mRNA的转录活性显著降低。PBK角膜和健康角膜中TGF-β1和TGF-β2的表达水平相同。
结论
TGF-β3基因表达下调可能在大泡性角膜病变中观察到的分子变化中起重要作用。
Zotero 插件