Kimsa-Dudek Magdalena, Kruszniewska-Rajs Celina, Krawczyk Agata, Grzegorczyk Anna, Synowiec-Wojtarowicz Agnieszka, Gola Joanna
Department of Nutrigenomics and Bromatology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jednosci 8, Sosnowiec, 41-200, Poland.
Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jednosci 8, Sosnowiec, 41-200, Poland.
Appl Biochem Biotechnol. 2025 Mar;197(3):1516-1533. doi: 10.1007/s12010-024-05094-z. Epub 2024 Nov 25.
Due to their properties, numerous polyphenols and a static magnetic field could have therapeutic potential. Therefore, the aim of our research was to investigate the effect of caffeic acid (CA), a moderate-strength static magnetic field (SMF) and their simultaneous action on human fibroblasts in order to determine the molecular pathways they affect, which might contribute to their potential use in therapeutic strategies. The research was conducted using normal human dermal fibroblasts (NHDF cells) that had been treated with caffeic acid at a concentration of 1 mmol/L and then exposed to a moderate-strength static magnetic field. The RNA that had been extracted from the collected cells was used as a template for next-generation sequencing (NGS) and an RT-qPCR reaction. We identified a total of 1,006 differentially expressed genes between CA-treated and control cells. Exposure of cells to a SMF altered the expression of only 99 genes. Simultaneous exposure to both factors affected the expression of 953 genes. It has also been shown that these genes mainly participate in cellular processes, including apoptosis. The highest fold change value were observed for HSPA6 and HSPA7 genes. In conclusion, the results of our research enabled the modulators, primarily caffeic acid and to a lesser extent a static magnetic field, of the apoptosis signaling pathway in human fibroblasts to be identified and to propose a mechanism of their action, which might be useful in the development of new preventive and/or therapeutic strategies. However, more research using other cell lines is needed including cancer cells.
由于其特性，众多多酚和静磁场可能具有治疗潜力。因此，我们研究的目的是探究咖啡酸（CA）、中等强度静磁场（SMF）及其联合作用对人成纤维细胞的影响，以确定它们所影响的分子途径，这可能有助于它们在治疗策略中的潜在应用。该研究使用正常人类皮肤成纤维细胞（NHDF细胞）进行，这些细胞用浓度为1 mmol/L的咖啡酸处理，然后暴露于中等强度静磁场。从收集的细胞中提取的RNA用作下一代测序（NGS）和RT-qPCR反应的模板。我们共鉴定出CA处理细胞和对照细胞之间有1006个差异表达基因。细胞暴露于SMF仅改变了99个基因的表达。两种因素同时暴露影响了953个基因的表达。还表明这些基因主要参与细胞过程，包括细胞凋亡。HSPA6和HSPA7基因的倍数变化值最高。总之，我们的研究结果能够鉴定出人成纤维细胞中细胞凋亡信号通路的调节剂，主要是咖啡酸，其次是静磁场，并提出它们的作用机制，这可能有助于开发新的预防和/或治疗策略。然而，需要使用包括癌细胞在内的其他细胞系进行更多研究。
