Izuta Hiroshi, Matsunaga Nozomu, Shimazawa Masamitsu, Sugiyama Tetsuya, Ikeda Tsunehiko, Hara Hideaki
Department of Biofunctional Evaluation, Molecular Pharmacology, Gifu Pharmaceutical University, Gifu, Japan.
Mol Vis. 2010 Jan 29;16:130-6.
To investigate the relationships among antioxidant activities, oxidative stress, and vascular endothelial growth factor (VEGF) in the vitreous body and serum from proliferative diabetic retinopathy (PDR) patients.
In 21 patients with PDR and 21 controls with macular hole (MH), the VEGF and lipid peroxide (Nepsilon-hexanoyl-lysine [HEL]) levels in the vitreous and serum were measured by enzyme-linked immunosorbent assay, while antioxidant capacity (potential antioxidant [PAO]) was measured by chemical reduction of Cu(2+).
Both the PAO and HEL levels in the vitreous and serum were significantly higher in PDR patients than in those with MH (both p<0.01). The VEGF concentrations in the vitreous were higher in PDR patients than in those with MH (p<0.01); however, the VEGF concentrations in the serum were not different between the two groups (p=0.95). Positive correlations were found between the PAO and VEGF concentrations and between the HEL and VEGF concentrations in the vitreous of both the PDR and the MH patients.
Our study revealed that the PAO, HEL, and VEGF concentrations in the vitreous were increased in PDR versus MH patients and that there were positive correlations among these factors. This is consistent with VEGF and lipid peroxide levels in the vitreous playing some role in the pathogenesis of PDR.
研究增殖性糖尿病视网膜病变（PDR）患者玻璃体和血清中的抗氧化活性、氧化应激与血管内皮生长因子（VEGF）之间的关系。
对21例PDR患者和21例黄斑裂孔（MH）对照者，采用酶联免疫吸附测定法测量玻璃体和血清中的VEGF及脂质过氧化物（Nε-己酰赖氨酸[HEL]）水平，同时通过化学还原Cu(2+)测量抗氧化能力（潜在抗氧化剂[PAO]）。
PDR患者玻璃体和血清中的PAO及HEL水平均显著高于MH患者（均p<0.01）。PDR患者玻璃体中的VEGF浓度高于MH患者（p<0.01）；然而，两组患者血清中的VEGF浓度无差异（p=0.95）。在PDR患者和MH患者的玻璃体中，PAO与VEGF浓度之间以及HEL与VEGF浓度之间均呈正相关。
我们的研究表明，与MH患者相比，PDR患者玻璃体中的PAO、HEL和VEGF浓度升高，且这些因素之间存在正相关。这与玻璃体中的VEGF和脂质过氧化物水平在PDR发病机制中发挥一定作用是一致的。
