炎症与糖尿病视网膜病变。
Inflammation and diabetic retinopathy.
机构信息
Ankara City Hospital, Associate Professor Doctor of Ophtalmology, Ankara, Turkey.
Ankara City Hospital, MD, Ophthalmologist, Ankara, Turkey.
出版信息
Mol Vis. 2020 Oct 15;26:718-721. eCollection 2020.
PURPOSE
To investigate the relationship between inflammation in the vitreous and diabetic retinopathy.
METHODS
Vitreous samples from 21 patients with proliferative diabetic retinopathy (PDR), 21 patients with nonproliferative diabetic retinopathy (NPDR), and 21 nondiabetic patients with idiopathic epiretinal membranes (control) were studied. The interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), matrix metalloproteinase (MMP)-2, MMP-9, and adiponectin levels in the vitreous were detected in all samples with enzyme-linked immunosorbent assay (ELISA). Samples were stored at -80 °C until analyzed.
RESULTS
The TNF-α levels in the vitreous were not statistically significant between all groups (p>0.005). The mean IFN-γ levels were statistically significantly higher in patients with PDR (70.98 pg/ml) and patients with NPDR (46.61 pg/ml) than in nondiabetic patients (22.02 pg/ml). There was a difference in the IFN-γ levels in the vitreous between patients with PDR and patients with NPDR (p<0.005). The MMP-2 and MMP-9 concentrations in the vitreous were not different between all groups (p>0.05). There was a correlation between the IFN-γ and TNF-α levels. We investigated the statistically significantly decreased levels of adiponectin in the proliferative (p<0.05) and nonproliferative (p<0.05) diabetic eyes compared to the nondiabetic eyes.
CONCLUSIONS
Increased levels of IFN-γ and TNF-α in the vitreous were found in patients with diabetes compared to nondiabetic patients. Decreased levels of adiponectin in the vitreous were found in patients with diabetes compared to nondiabetic patients. The data support the hypothesis that inflammation is associated with diabetic retinopathy.
目的
研究玻璃体炎症与糖尿病视网膜病变的关系。
方法
研究了 21 例增殖性糖尿病视网膜病变（PDR）、21 例非增殖性糖尿病视网膜病变（NPDR）和 21 例特发性视网膜内界膜（对照组）患者的玻璃体样本。采用酶联免疫吸附试验（ELISA）检测所有样本中玻璃体干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）和脂联素水平。标本于-80℃保存，待分析。
结果
各组玻璃体 TNF-α水平无统计学差异（p＞0.005）。PDR 患者（70.98pg/ml）和 NPDR 患者（46.61pg/ml）玻璃体 IFN-γ水平明显高于非糖尿病患者（22.02pg/ml）。PDR 患者和 NPDR 患者玻璃体 IFN-γ水平存在差异（p＜0.005）。各组玻璃体 MMP-2 和 MMP-9 浓度无差异（p＞0.05）。IFN-γ与 TNF-α水平存在相关性。我们发现增殖性（p＜0.05）和非增殖性（p＜0.05）糖尿病眼中的脂联素水平明显低于非糖尿病眼。
结论
与非糖尿病患者相比，糖尿病患者玻璃体中 IFN-γ和 TNF-α水平升高。与非糖尿病患者相比，糖尿病患者玻璃体中脂联素水平降低。这些数据支持炎症与糖尿病视网膜病变有关的假说。
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