Ray Ramesh Chandra, Sharma Piyush, Panda Smita Hasini
Regional Centre of Central Tuber Crops Research Institute, Bhubaneswar 751 019, India.
J Environ Biol. 2009 Sep;30(5 Suppl):847-52.
During extraction of starch from cassava, fibrous residue is a major waste released into the environment. Owing to the high starch content (60-65% on dry weight basis) and organic matter of cassava fibrous residue (CFR), an attempt has been made to utilize it for the production of lactic acid (LA) in semi solid state fermentation using Mann Rogassa Sharpe medium containing [5 % (wv(-1))] CFR in lieu of glucose [2% (wv(-1))] as the carbon source. Response Surface Methodology (RSM) was used to evaluate the effect of main variables, i.e. incubation period, temperature and pH on LA production. The experimental results showed that the optimum incubation period, temperature and pH were 120 hr 35 degrees C and 6.5, respectively. Maximum starch conversion by Lactobacillus plantarum MTCC 1407 to LA was 63.3%. The organism produced 29.86 g of (L+) LA from 60 g of starch present in 100 g of CFR. The LA production yield (i.e. mass LA produced mass starch present in CFR(-1) x 100) was 49.76%.
在从木薯中提取淀粉的过程中，纤维残渣是排放到环境中的主要废弃物。由于木薯纤维残渣（CFR）的淀粉含量高（以干重计为60 - 65%）且含有有机物，因此尝试利用其在半固态发酵中生产乳酸（LA），使用含有[5%（w/v⁻¹）]CFR的曼罗加萨夏普培养基代替[2%（w/v⁻¹）]葡萄糖作为碳源。采用响应面法（RSM）评估主要变量，即培养时间、温度和pH对LA产量的影响。实验结果表明，最佳培养时间、温度和pH分别为120小时、35℃和6.5。植物乳杆菌MTCC 1407将淀粉转化为LA的最大转化率为63.3%。该微生物从100克CFR中所含的60克淀粉中产生了29.86克（L +）LA。LA的生产产率（即产生的LA质量/CFR中存在的淀粉质量⁻¹×100）为49.76%。
