Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian 350108, China.
J Agric Food Chem. 2010 Mar 10;58(5):2763-70. doi: 10.1021/jf903274z.
Auxin plays an important role in cell differentiation, apical dominance, and tropism in plants. A new method based on dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography-fluorescence detection (HPLC-FLD) has been established to detect auxin. A mixture of CHCl(3) (extraction solvent) and acetone (disperser solvent) was injected quickly into a sample solution with desired salt concentration and pH value, and then a cloudy solution consisting of many dispersed fine droplets of CHCl(3) was formed. After centrifugation, the sedimented phase was withdrawn and directly analyzed by HPLC-FLD. Under optimal conditions, four auxins were baseline separated within 3.5 min, with the minimal limit of detection of 0.02 ng mL(-1) and coefficient correlations in the range of 0.9980-0.9995. This simple method was successfully applied to real sample analysis. Experimental results showed that DLLME was a high-performance and powerful preconcentration method to extract and enrich related plant auxin.
植物中的生长素在细胞分化、顶端优势和向性中起着重要作用。建立了一种基于分散液-液微萃取（DLLME）与高效液相色谱-荧光检测（HPLC-FLD）相结合的新方法来检测生长素。将一定浓度的盐和 pH 值的样品溶液与氯仿（萃取溶剂）和丙酮（分散溶剂）的混合物快速注入，然后形成由许多分散的氯仿小液滴组成的混浊溶液。离心后，取出沉淀相并直接通过 HPLC-FLD 进行分析。在最佳条件下，四种生长素在 3.5 分钟内实现基线分离，最小检测限为 0.02ng/mL，相关系数在 0.9980-0.9995 范围内。该简单方法成功应用于实际样品分析。实验结果表明，DLLME 是一种高性能、强大的浓缩方法，可用于提取和富集相关的植物生长素。
