采用分散液液微萃取结合高效液相色谱荧光检测法提取和分析植物中的植物生长素。
Extraction and analysis of auxins in plants using dispersive liquid-liquid microextraction followed by high-performance liquid chromatography with fluorescence detection.
机构信息
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Analytical and Testing Center, The Sport Science Research Center, Fuzhou University, Fuzhou, Fujian 350108, China.
出版信息
J Agric Food Chem. 2010 Mar 10;58(5):2763-70. doi: 10.1021/jf903274z.
Auxin plays an important role in cell differentiation, apical dominance, and tropism in plants. A new method based on dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography-fluorescence detection (HPLC-FLD) has been established to detect auxin. A mixture of CHCl(3) (extraction solvent) and acetone (disperser solvent) was injected quickly into a sample solution with desired salt concentration and pH value, and then a cloudy solution consisting of many dispersed fine droplets of CHCl(3) was formed. After centrifugation, the sedimented phase was withdrawn and directly analyzed by HPLC-FLD. Under optimal conditions, four auxins were baseline separated within 3.5 min, with the minimal limit of detection of 0.02 ng mL(-1) and coefficient correlations in the range of 0.9980-0.9995. This simple method was successfully applied to real sample analysis. Experimental results showed that DLLME was a high-performance and powerful preconcentration method to extract and enrich related plant auxin.
植物中的生长素在细胞分化、顶端优势和向性中起着重要作用。建立了一种基于分散液-液微萃取（DLLME）与高效液相色谱-荧光检测（HPLC-FLD）相结合的新方法来检测生长素。将一定浓度的盐和 pH 值的样品溶液与氯仿（萃取溶剂）和丙酮（分散溶剂）的混合物快速注入，然后形成由许多分散的氯仿小液滴组成的混浊溶液。离心后，取出沉淀相并直接通过 HPLC-FLD 进行分析。在最佳条件下，四种生长素在 3.5 分钟内实现基线分离，最小检测限为 0.02ng/mL，相关系数在 0.9980-0.9995 范围内。该简单方法成功应用于实际样品分析。实验结果表明，DLLME 是一种高性能、强大的浓缩方法，可用于提取和富集相关的植物生长素。
Zotero 插件