Feng Xiaoxiao, Yu Jianlei, Pan Lixiang, Song Guochun, Zhang Hongyan
College of Science, China Agricultural University, Beijing 100193, China.
Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Int J Environ Res Public Health. 2016 May 26;13(6):534. doi: 10.3390/ijerph13060534.
Dichlorprop-P and bentazone have been widely used in the prevention and control of weeds in wheat field ecosystems. There is a concern that pesticide residues and metabolites remain on or in the wheat. Thus, the study of the determination and monitoring of their residues in wheat has important significance. A rapid, simple and reliable QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method was modified, developed and validated for the determination of dichlorprop-P, bentazone and its metabolites (6-hydroxy-bentazone and 8-hydroxy-bentazone) in wheat (wheat plants, wheat straw and grains of wheat) using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The average recoveries of this method ranged from 72.9% to 108.7%, and the limits of quantification (LOQs) were 2.5-12 μg/kg. The dissipation and final residue of four compounds in three provinces (Shandong, Jiangsu and Heilongjiang) in China were studied. The trial results showed that the half-lives of dichlorprop-P and bentazone were 1.9-2.5 days and 0.5-2.4 days in wheat plants, respectively. The terminal residues in grains of wheat and wheat straw at harvest were all much below the maximum residue limit (MRL) of 0.2 mg/kg for dichlorprop-P and 0.1 mg/kg for bentazone established by the European Union (EU, Regulation No. 396/2005).
二氯丙酸 - P和灭草松已广泛用于麦田生态系统中杂草的防治。人们担心农药残留及其代谢物会残留在小麦上或小麦中。因此，研究小麦中它们残留量的测定和监测具有重要意义。本文改进、开发并验证了一种快速、简单且可靠的QuEChERS（快速、简便、廉价、高效、耐用且安全）方法，用于采用高效液相色谱 - 串联质谱法（HPLC - MS/MS）测定小麦（小麦植株、麦秸和麦粒）中的二氯丙酸 - P、灭草松及其代谢物（6 - 羟基 - 灭草松和8 - 羟基 - 灭草松）。该方法的平均回收率在72.9%至108.7%之间，定量限（LOQs）为2.5 - 12μg/kg。研究了这四种化合物在中国三个省份（山东、江苏和黑龙江）的消解情况及最终残留量。试验结果表明，二氯丙酸 - P和灭草松在小麦植株中的半衰期分别为1.9 - 2.5天和0.5 - 2.4天。收获时麦粒和麦秸中的最终残留量均远低于欧盟（EU，法规编号396/2005）规定的二氯丙酸 - P最大残留限量（MRL）0.2mg/kg和灭草松最大残留限量0.1mg/kg。
