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在处女牛乳腺的炎症反应之前和期间,常驻和炎性巨噬细胞的凋亡。

Apoptosis of resident and inflammatory macrophages before and during the inflammatory response of the virgin bovine mammary gland.

机构信息

Department of Morphology, Physiology and Animal Genetics, Mendel University, Zemedelska 1, 613 00 Brno, Czech Republic.

出版信息

Acta Vet Scand. 2010 Feb 9;52(1):12. doi: 10.1186/1751-0147-52-12.

Abstract

BACKGROUND

Macrophages may play a prominent role in defense of the bovine mammary gland, and their functionality is necessary for successful eradication of bacterial pathogens. In contrast to necrosis, however, apoptosis has not yet been studied in macrophages from bovine mammary glands. Therefore, the aim of this study was to confirm the occurrence of apoptosis in macrophages from resting heifer mammary glands and during the inflammatory response.

METHODS

Inflammatory response was induced by phosphate buffered saline (PBS) and by lipopolysaccharide (LPS). Resident macrophages (RESMAC) were obtained before and inflammatory macrophages (INFMAC) 24, 48, 72 and 168 hours after inducing inflammatory response in mammary glands of unbred heifers. Cell samples were analyzed for differential counts, apoptosis and necrosis using flow cytometry.

RESULTS

Populations of RESMAC and INFMAC contained monocyte-like cells and vacuolized cells. Apoptosis was detected differentially in both morphologically different types of RESMAC and INFMAC and also during initiation and resolution of the inflammatory response. In the RESMAC population, approximately one-tenth of monocyte-like cells and one-third of vacuolized cells were apoptotic. In the INFMAC population obtained 24 h after PBS treatment, approximately one-tenth of monocyte-like cells and almost one-quarter of vacuolized cells were apoptotic. At the same time following LPS, however, we observed a significantly lower percentage of apoptotic cells in the population of monocyte-like INFMAC and vacuolized INFMAC. Moreover, a higher percentage of apoptotic cells in INFMAC was detected during all time points after PBS in contrast to LPS. Comparing RESMAC and INFMAC, we observed that vacuolized cells from populations of RESMAC and INFMAC underwent apoptosis more intensively than did monocyte-like cells.

CONCLUSIONS

We conclude that apoptosis of virgin mammary gland macrophages is involved in regulating their lifespan, and it is involved in the resolution process of the inflammatory response.

摘要

背景

巨噬细胞在奶牛乳腺的防御中可能发挥着重要作用,其功能对于成功消除细菌病原体是必要的。然而,与坏死不同,凋亡尚未在奶牛乳腺的巨噬细胞中进行研究。因此,本研究的目的是确认静息奶牛乳腺中的巨噬细胞以及在炎症反应过程中是否发生凋亡。

方法

通过磷酸盐缓冲盐水(PBS)和脂多糖(LPS)诱导炎症反应。在诱导未繁殖奶牛乳腺的炎症反应之前和之后 24、48、72 和 168 小时,获得常驻巨噬细胞(RESMAC)和炎症巨噬细胞(INFMAC)。使用流式细胞术分析细胞样品的差异计数、凋亡和坏死。

结果

RESMAC 和 INFMAC 群体包含单核样细胞和空泡化细胞。在两种形态不同的 RESMAC 和 INFMAC 以及在炎症反应的起始和解决过程中,均检测到凋亡的差异。在 RESMAC 群体中,约十分之一的单核样细胞和三分之一的空泡化细胞发生凋亡。在 PBS 处理后 24 小时获得的 INFMAC 群体中,约十分之一的单核样细胞和约四分之一的空泡化细胞发生凋亡。然而,在 LPS 处理后同一时间,我们观察到单核样 INFMAC 和空泡化 INFMAC 群体中的凋亡细胞比例明显较低。此外,与 LPS 相比,在 PBS 后所有时间点都检测到 INFMAC 中凋亡细胞的比例较高。比较 RESMAC 和 INFMAC,我们观察到 RESMAC 和 INFMAC 群体中的空泡化细胞比单核样细胞更容易发生凋亡。

结论

我们得出结论,即处女期乳腺巨噬细胞的凋亡参与调节其寿命,并参与炎症反应的解决过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7af/2829577/a18f12610517/1751-0147-52-12-1.jpg

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