脂多糖对牛乳腺巨噬细胞功能的影响。
The effect of lipopolysaccharide on bovine mammary macrophage function.
作者信息
Politis I, Zhao X, McBride B W, Burton J H
机构信息
Department of Animal and Poultry Science, University of Guelph, Ontario.
出版信息
Can J Vet Res. 1991 Jul;55(3):220-3.
The effect of Escherichia coli lipopolysaccharide (LPS) on the expression of major histocompatibility complex (MHC) class II molecules by bovine mammary macrophages was examined. The ability of LPS-treated mammary macrophages to support antigen-specific T-cell proliferation, as a measure of their antigen presentation ability, was also evaluated. For this purpose, control and LPS-treated macrophages were pulsed with heat-killed Staphylococcus aureus and then cultured with S. aureus-sensitized T-cells. Our data show that LPS had no significant effect on the expression of MHC class II molecules on the surface of mammary macrophages. Furthermore, LPS-induced macrophages were no more active in supporting T-cell proliferation on a per cell basis than unstimulated macrophages. The lack of macrophage response to LPS with respect to expression of MHC class II molecules and the antigen presentation ability is another example of the hyporesponsive nature of macrophages isolated from the bovine mammary gland.
研究了大肠杆菌脂多糖（LPS）对牛乳腺巨噬细胞主要组织相容性复合体（MHC）II类分子表达的影响。还评估了经LPS处理的乳腺巨噬细胞支持抗原特异性T细胞增殖的能力，以此作为其抗原呈递能力的指标。为此，用热灭活的金黄色葡萄球菌刺激对照巨噬细胞和经LPS处理的巨噬细胞，然后与经金黄色葡萄球菌致敏的T细胞共同培养。我们的数据表明，LPS对乳腺巨噬细胞表面MHC II类分子的表达没有显著影响。此外，就单个细胞而言，LPS诱导的巨噬细胞在支持T细胞增殖方面并不比未刺激的巨噬细胞更活跃。从牛乳腺分离的巨噬细胞对LPS在MHC II类分子表达和抗原呈递能力方面缺乏反应，是巨噬细胞低反应性的又一个例子。
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