Li Dong-Ri, Ishikawa Takaki, Quan Li, Zhao Dong, Michiue Tomomi, Zhu Bao-Li, Wang Hui Jun, Maeda Hitoshi
Department of Legal Medicine, Osaka City University Medical School, Asahi-machi 1-4-3, Abeno, Osaka 545-8558, Japan.
Leg Med (Tokyo). 2010 Mar;12(2):63-7. doi: 10.1016/j.legalmed.2009.11.005. Epub 2010 Feb 9.
The present study investigated the morphology of astrocytes in the hippocampus and serum S100B levels in cases of mechanical asphyxia due to neck compression (n=23: atypical hanging, n=7; ligature/manual strangulation, n=16) with regard to the classical autopsy findings, compared with those of other types of asphyxiation (n=9) and acute myocardial infarction/ischemia (AMI, n=20). The decrease in intact astrocyte number, as shown by S100 and GFAP-immunostaining, was larger for asphyxiation due to neck compression compared with that for other asphyxiation and AMI, showing a correlation with the increase in the serum S100B levels. The decrease in intact astrocyte number and increase in serum S100B were closely related to the severity of conjunctival petechial hemorrhage and fracture(s) of the hyoid bone and/or thyroid cartilage in asphyxia due to neck compression. These findings suggest that hippocampal astrocyte injury is caused by cerebral hypoxia accompanied by congestion, especially in mechanical asphyxia due to neck compression.
本研究调查了颈部受压导致机械性窒息（n = 23：非典型缢死，n = 7；勒颈/手扼颈，n = 16）病例中，海马体星形胶质细胞的形态以及血清S100B水平，并结合经典尸检结果，与其他类型的窒息（n = 9）和急性心肌梗死/缺血（AMI，n = 20）病例进行比较。S100和GFAP免疫染色显示，与其他窒息和AMI相比，颈部受压导致的窒息中完整星形胶质细胞数量的减少更为明显，且与血清S100B水平的升高相关。在颈部受压导致的窒息中，完整星形胶质细胞数量的减少和血清S100B的升高与结膜点状出血的严重程度以及舌骨和/或甲状软骨骨折密切相关。这些发现表明，海马体星形胶质细胞损伤是由伴有充血的脑缺氧引起的，尤其是在颈部受压导致的机械性窒息中。
