蓝藻毒素柱孢藻肝毒素诱导叙利亚仓鼠胚胎（SHE）细胞形态转化。

Morphological cell transformation of Syrian hamster embryo (SHE) cells by the cyanotoxin, cylindrospermopsin.

机构信息

Laboratoire des Interactions Ecotoxicologie, Biodiversité, Ecosystèmes (LIEBE), Université Paul Verlaine de METZ, UMR CNRS 7146, Rue du Général Delestraint, 57070 Metz, France.

出版信息

Toxicon. 2010 Jun 15;55(7):1317-22. doi: 10.1016/j.toxicon.2010.01.025. Epub 2010 Feb 6.

DOI:10.1016/j.toxicon.2010.01.025

PMID:20144639

Abstract

Cylindrospermopsin (CYN) is a cyanotoxin which has been implicated in human intoxication and animal mortality. Genotoxic activity of this hepatotoxin is known but its carcinogenic activity remains to be elucidated. In this work, CYN was assessed for its cell-transforming activity using the Syrian hamster embryo (SHE) cell transformation assay. This in vitro assay is used to evaluate the carcinogenic potential of chemical, physical and biological agents in SHE cells, which are primary, normal, diploid, genetically stable and capable of metabolic activation. We demonstrated that CYN induced a significant increase in morphological cell transformation in SHE cells following a 7-day continuous treatment in the range of non-cytotoxic concentrations 1 x 10(-7)-1 x 10(-2) ng/mL.

摘要

柱孢藻毒素（CYN）是一种蓝藻毒素，已被证实与人类中毒和动物死亡有关。这种肝毒素具有遗传毒性活性，但致癌活性仍有待阐明。在这项工作中，我们使用叙利亚仓鼠胚胎（SHE）细胞转化试验来评估 CYN 的细胞转化活性。该体外试验用于评估化学、物理和生物因子在 SHE 细胞中的致癌潜力，SHE 细胞是原代、正常、二倍体、遗传稳定且具有代谢激活能力的细胞。我们证明，在 1 x 10(-7)-1 x 10(-2) ng/mL 的非细胞毒性浓度范围内，CYN 对 SHE 细胞进行为期 7 天的连续处理后，可显著诱导形态学细胞转化。