Guerrero L, Sarasa M, López Y, Soy D
Servei de Farmacia, Hospital Clínic de Barcelona, Barcelona, Spain.
Farm Hosp. 2010 Jan-Feb;34(1):27-31. doi: 10.1016/j.farma.2009.07.001.
Evaluation of an analytic method for determining linezolid concentrations in biological fluids including plasma, vitreous humour and cerebrospinal fluid using high-efficiency liquid chromatography and subsequent ultraviolet detection.
The method was validated by studying the following parameters: accuracy, precision, sensitivity, linearity and recovery. The drug was extracted from the biological matrix by means of a protein precipitation with perchloric acid. Chromatographic separation was performed by eluting linezolid with a mobile phase consisting of 80% K2HPO4 buffer solution (15 mM; pH=5) and 20% acetonitrile, and a stationary phase, NOVAPAK C18 150x3.9 mm with precolumn. The wavelength reading was 254 nm and the working flow rate was 1 ml/min.
We obtained values with accuracies between 94.4 and 106.1%, and precisions between 0.88-6% and 3.7-5.6% for intra-and inter-day variability, respectively. Recovery obtained after analysing the plasma samples was at 93%. The method showed itself to be linear for the concentration levels under study.
The method's behaviour can be described as linear, precise and accurate. Furthermore, the method is fast, sensitive, and inexpensive. It is useful for determining linezolid concentrations in multiple biological matrices. It can also be used as a basis for further clinical pharmacokinetic studies.
评估一种使用高效液相色谱及后续紫外检测法测定生物体液（包括血浆、玻璃体液和脑脊液）中利奈唑胺浓度的分析方法。
通过研究以下参数验证该方法：准确度、精密度、灵敏度、线性和回收率。采用高氯酸蛋白沉淀法从生物基质中提取药物。色谱分离是用由80% K2HPO4缓冲溶液（15 mM；pH = 5）和20%乙腈组成的流动相洗脱利奈唑胺，并使用带预柱的NOVAPAK C18 150x3.9 mm固定相。波长读数为254 nm，工作流速为1 ml/min。
我们获得的准确度值在94.4%至106.1%之间，日内和日间变异性的精密度分别在0.88 - 6%和3.7 - 5.6%之间。分析血浆样品后获得的回收率为93%。该方法在所研究的浓度水平下呈线性。
该方法的性能可描述为线性、精密且准确。此外，该方法快速、灵敏且成本低廉。它可用于测定多种生物基质中的利奈唑胺浓度。它还可作为进一步临床药代动力学研究的基础。
