Key Laboratory of Medical Molecular Virology, Institute of Medical Microbiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Virus Res. 2010 May;149(2):245-51. doi: 10.1016/j.virusres.2010.02.002. Epub 2010 Feb 8.
Due to its high similarity to human hepatitis B virus (HBV), duck HBV (DHBV) is often used as an important model for HBV research. While inter-genotypic recombination of HBV is common, it has not been reported with DHBV. In this study, 32 non-redundant DHBV complete genomes were analyzed using phylogenetic methods and classified into two clusters, corresponding to the 'Chinese' and 'Western country' branches previously reported based on geographical distribution. One 'Chinese' branch strain was isolated in Australia and three 'Western country' branch strains were isolated in China, suggesting cross-geographical distribution of both branches. Recombination analyses of the 32 DHBV genomes identified two possible inter-genotypic recombination events with high confidence value. These recombination events occurred between the lineages represented, respectively, by the Chinese isolate GD3 (AY536371, 'Chinese' branch) and the American isolate DHBV16 (K01834, 'Western country' branch), giving rise to two Chinese recombinant isolates CH4 (EU429324) and CH6 (EU429326). The identification of inter-genotypic recombination among circulating DHBV isolates suggests the usefulness of DHBV as a model for studying the mechanism of HBV recombination.
由于其与人乙型肝炎病毒（HBV）高度相似，鸭乙型肝炎病毒（DHBV）常被用作乙型肝炎病毒研究的重要模型。虽然 HBV 的基因型间重组很常见，但尚未有关于 DHBV 的报道。在这项研究中，使用系统发育方法对 32 个非冗余的 DHBV 全基因组进行了分析，并分为两个聚类，与之前根据地理分布报道的“中国”和“西方国家”分支相对应。一个“中国”分支株在澳大利亚分离，三个“西方国家”分支株在中国分离，提示两个分支存在跨地理分布。对 32 个 DHBV 基因组的重组分析鉴定出两个具有高置信度值的可能的基因型间重组事件。这些重组事件发生在分别由中国分离株 GD3（AY536371，“中国”分支）和美国分离株 DHBV16（K01834，“西方国家”分支）代表的谱系之间，产生了两个中国重组分离株 CH4（EU429324）和 CH6（EU429326）。循环 DHBV 分离株中基因型间重组的鉴定表明，DHBV 可用作研究 HBV 重组机制的模型。
