Hu Quan, Zhang Xiaoyong, Lei Yangchang, Zhang Zhengmao, Mengji Lu, Yang Dongliang
Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):633-6. doi: 10.1007/s11596-006-0541-4.
The objective of this study was to characterize the genome structure of duck hepatitis B virus (DHBV) isolated from Hubei brown ducks. The natural carrier rate of DHBV in adult ducks from Hubei area was investigated and the DHBV DNA-positive serum screened out. The complete genome of a DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the carrier rate of DHBV in Hubei brown ducks was 10 %. This strain (GenBank accession number DQ276978) had a genome of 3024 nucleotides with three overlapping open reading frames encoding the surface, core and polymerase proteins respectively. Comparison of the strain with 17 DHBV strains registered in GenBank revealed a homology from 89.3 % to 93.5 % at the nucleotide level. The sequences of the structural and functional domains of these proteins were highly conserved. The strain was found to share more signature amino acids in the polymerase genes with the "Chinese" DHBV strains than those of the "Western" country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, the DQ276978 might belong to a subtype of the Chinese DHBV strains.
本研究的目的是对从湖北麻鸭分离出的鸭乙型肝炎病毒（DHBV）的基因组结构进行表征。调查了湖北地区成年鸭中DHBV的自然携带率，并筛选出DHBV DNA阳性血清。通过聚合酶链反应（PCR）扩增一株DHBV毒株的完整基因组，克隆至T载体并进行测序。结果显示，湖北麻鸭中DHBV的携带率为10%。该毒株（GenBank登录号DQ276978）基因组有3024个核苷酸，具有三个重叠的开放阅读框，分别编码表面蛋白、核心蛋白和聚合酶蛋白。将该毒株与GenBank中登记的17株DHBV毒株进行比较，核苷酸水平的同源性为89.3%至93.5%。这些蛋白的结构和功能域序列高度保守。发现该毒株在聚合酶基因中与“中国”DHBV毒株共享的特征性氨基酸比“西方”国家毒株更多。系统发育树分析也证实了这一发现。因此，DQ276978可能属于中国DHBV毒株的一个亚型。
