Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA.
J Cell Sci. 2010 Feb 15;123(Pt 4):519-28. doi: 10.1242/jcs.051326.
The working mechanism of the eukaryotic flagellar axoneme remains one of nature's most enduring puzzles. The basic mechanical operation of the axoneme is now a story that is fairly complete; however, the mechanism for coordinating the action of the dynein motor proteins to produce beating is still controversial. Although a full grasp of the dynein switching mechanism remains elusive, recent experimental reports provide new insights that might finally disclose the secrets of the beating mechanism: the special role of the inner dynein arms, especially dynein I1 and the dynein regulatory complex, the importance of the dynein microtubule-binding affinity at the stalk, and the role of bending in the selection of the active dynein group have all been implicated by major new evidence. This Commentary considers this new evidence in the context of various hypotheses of how axonemal dynein coordination might work.
真核鞭毛轴丝的工作机制仍然是自然界最持久的谜题之一。轴丝的基本机械操作现在已经是一个相当完整的故事；然而，协调动力蛋白马达蛋白的作用以产生运动的机制仍然存在争议。尽管对动力蛋白开关机制的全面理解仍然难以捉摸，但最近的实验报告提供了新的见解，这些见解可能最终揭示出运动机制的秘密：内动力蛋白臂的特殊作用，特别是动力蛋白 I1 和动力蛋白调节复合物，茎干处动力蛋白微管结合亲和力的重要性，以及弯曲在选择活性动力蛋白组中的作用，所有这些都被主要的新证据所暗示。本评论从轴丝动力蛋白协调可能的工作方式的各种假设的角度考虑了这一新证据。
