Källén R, Borgström A
Department of Surgery, Malmö General Hospital, University of Lund, Sweden.
Transplantation. 1991 Apr;51(4):754-9. doi: 10.1097/00007890-199104000-00003.
Biochemical signs of pancreatitis in plasma and pancreatic exudates were determined in 22 pigs subjected to pancreatic allograft transplantation after the graft had been in cold storage for 6 hr. Two perfusion and preservation media were used. We found signs of protease activation in the pancreatic exudate during the first hour after reperfusion. The local protease protection barrier was, however, not broken and no plasma changes indicating pancreatitis were seen during this period. On the first and second postoperative days, mild biochemical signs of pancreatitis were seen in the plasma, including a decrease in kininogen and C3 concentration as well as in plasma kallikrein inhibitory activity and the appearance of trypsin-protease inhibitor complexes. No correlation was seen between these biochemical signs of pancreatitis and graft appearance or function, indicating that the reperfusion pancreatitis seen after 6 hr of cold storage is of minor significance. No significant differences were seen between the two preservation media used (Perfadex and EuroCollins solution).
在22只接受胰腺同种异体移植的猪中，在移植物冷藏6小时后，测定血浆和胰腺渗出液中胰腺炎的生化指标。使用了两种灌注和保存介质。我们发现在再灌注后的第一个小时内，胰腺渗出液中有蛋白酶激活的迹象。然而，在此期间，局部蛋白酶保护屏障未被破坏，也未观察到表明胰腺炎的血浆变化。在术后第一天和第二天，血浆中出现了轻度胰腺炎的生化指标，包括激肽原和C3浓度降低，血浆激肽释放酶抑制活性降低以及胰蛋白酶 - 蛋白酶抑制剂复合物的出现。这些胰腺炎的生化指标与移植物外观或功能之间没有相关性，表明冷藏6小时后出现的再灌注性胰腺炎意义不大。所使用的两种保存介质（Perfadex和EuroCollins溶液）之间没有显著差异。
