Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Wołyńska 35, 60-637 Poznań, Poland.
J Appl Genet. 2010;51(1):33-5. doi: 10.1007/BF03195708.
Wild pear (Pyrus pyraster, syn. P. communis var. pyraster) is thought to be one of the species that gave rise to all other members of the genus Pyrus, although intraspecific hybridizations with cultivated varieties could cause the disappearance of original species characteristics. S-RNase alleles from 7 different wild pear individuals, collected from various regions of Poland, were cloned on the basis of the PCR method and nucleotide sequence analyses. The hypervariable (HV) region is responsible for allele-specific S-RNase activity in the self-incompatibility mechanism. The high level of polymorphism of its sequences may constitute a source of valuable phylogenetic information. From all individuals, 14 sequences were obtained successfully, and 9 of them were novel alleles. Phylogenetic analysis of these alleles was based on the amino acid sequence interpretation of coding regions and intron nucleotide sequences. The research conducted on a limited pool of available P. pyraster alleles gives only an initial insight into possible S-RNase allele polymorphisms in wild populations. At this stage, the results do not confirm a strong influence of cultivated pear species on the wild pear.
沙梨（Pyrus pyraster，同义词 P. communis var. pyraster）被认为是所有梨属物种的起源之一，尽管与栽培品种的种内杂交可能导致原始物种特征的消失。从波兰不同地区采集的 7 个不同野生梨个体的 S-RNase 等位基因，基于 PCR 方法和核苷酸序列分析进行了克隆。高变区（HV）负责自交不亲和机制中等位基因特异性 S-RNase 活性。其序列的高度多态性可能构成有价值的系统发育信息来源。从所有个体中成功获得了 14 个序列，其中 9 个是新等位基因。这些等位基因的系统发育分析基于编码区和内含子核苷酸序列的氨基酸序列解释。对有限数量的可用 P. pyraster 等位基因的研究仅初步了解了野生种群中可能存在的 S-RNase 等位基因多态性。在现阶段，结果并不能证实栽培梨品种对野生梨的强烈影响。
