用任意引物扩增的DNA多态性可作为遗传标记。
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
作者信息
Williams J G, Kubelik A R, Livak K J, Rafalski J A, Tingey S V
机构信息
Central Research and Development Department, E.I. du Pont de Nemours & Co., Inc, Wilmington, DE 19880.
出版信息
Nucleic Acids Res. 1990 Nov 25;18(22):6531-5. doi: 10.1093/nar/18.22.6531.
Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
分子遗传图谱通常是通过分析有性杂交后代中限制性片段长度多态性（RFLP）的分离情况来构建的。在此，我们描述了一种基于用任意核苷酸序列的单引物扩增随机DNA片段的新DNA多态性检测方法。这些多态性，简单地检测为从一个亲本而非另一个亲本扩增出的DNA片段，以孟德尔方式遗传，可用于构建多种物种的遗传图谱。我们建议将这些多态性称为RAPD标记，即随机扩增多态性DNA。
Zotero 插件