Aras Sumer, Kanlitepe Ciğdem, Cansaran-Duman Demet, Halici Mehmet Gökhan, Beyaztaş Taylan
Biotechnology Section, Department of Biology, Faculty of Science, University of Ankara, Tandogan, 06100, Ankara, Turkey.
J Environ Monit. 2010 Feb;12(2):536-43. doi: 10.1039/b906717e. Epub 2009 Nov 18.
In living organisms heterogenous air pollutants are considered as a major source of DNA damage. In this study, we aimed to describe the DNA changes in Pseudevernia furfuracea (L.) Zopf samples exposed to pollution at various sites in Kayseri by RAPD (random amplified polymorphic DNA) analysis in order to reveal the pattern of genetic variation influenced by the environmental pollution. The study area is characterized by the presence of numerous industrial activities, such as steel works, glassworks, shipbuilding, and metallurgical, mechanical, chemical and food industries. Control samples were collected from Cat forest (Sivas) and exposed to pollution in dry and wet seasons in the province of Kayseri located in Central Anatolia. Results indicated that heterogeneous pollutants might have contributed to the changes in the band patterns obtained by RAPD analysis, reflecting the occurrence of DNA damage in the control samples.
在生物体内，异质空气污染物被认为是DNA损伤的主要来源。在本研究中，我们旨在通过RAPD（随机扩增多态性DNA）分析描述在开塞利不同地点受污染的淡黄粉衣样本中的DNA变化，以揭示受环境污染影响的遗传变异模式。研究区域的特点是存在众多工业活动，如钢铁厂、玻璃厂、造船厂以及冶金、机械、化工和食品工业。对照样本采自卡特森林（锡瓦斯），并在位于安纳托利亚中部的开塞利省的干湿季节接受污染。结果表明，异质污染物可能导致了RAPD分析所获得的条带模式的变化，这反映了对照样本中DNA损伤的发生。
