Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
Appl Microbiol Biotechnol. 2010 Mar;86(2):445-67. doi: 10.1007/s00253-009-2303-2. Epub 2010 Feb 10.
L-methioninase is ubiquitous in all organisms except in mammals. It mainly catalyzes the, alpha, gamma-elimination of L: -methionine to alpha-ketobutyrate, methanethiol, and ammonia. Unlike normal cells, methionine dependency was reported as a biochemical phenomenon among various types of cancer cells. Thus, L-methioninase is the universal protocol for triggering the majority of tumor cells. This review is an attempt to briefly describe the occurrence of the biochemical and molecular properties of L-methioninase by a comparative manner to the eukaryotic and prokaryotic source for the maximum exploitation in the therapeutic field. The combination of L-methioninase treatment, gene therapy, and chemotherapeutic drugs clearly explores the various therapeutic aspects of this enzyme. Finally, the perspectives for increasing the therapeutic efficacy of this enzyme were described.
L-甲硫氨酸酶普遍存在于除哺乳动物以外的所有生物体中。它主要催化 L：-甲硫氨酸的α，γ-消除反应，生成α-酮丁酸、甲硫醇和氨。与正常细胞不同，各种类型的癌细胞中都报道了依赖蛋氨酸作为生化现象。因此，L-甲硫氨酸酶是触发大多数肿瘤细胞的通用方案。本综述试图通过与真核生物和原核生物来源的比较方式，简要描述 L-甲硫氨酸酶的生化和分子特性的发生，以最大限度地开发其在治疗领域的应用。L-甲硫氨酸酶治疗、基因治疗和化疗药物的联合使用清楚地探索了这种酶的各种治疗方面。最后，还描述了提高该酶治疗效果的前景。
