Horns T, Jeske H
Institut für Allgemeine Botanik, Angewandte Molekularbiologie der Pflanzen, Hamburg, Germany.
Virology. 1991 Apr;181(2):580-8. doi: 10.1016/0042-6822(91)90891-e.
Abutilon mosaic virus (AbMV) is a whitefly-transmitted geminivirus with a a bipartite genome. Using in situ hybridization AbMV DNA was detected exclusively in the phloem of infected Abutilon sellovianum leaves and intracellularly predominant in the nuclei. No AbMV DNA was found in cells from palisade and spongy parenchyma, the tissues which show the predominant cytopathological effects. A hypothesis is discussed to account for the finding that, while AbMV accumulates in the phloem, symptoms are observed in other tissues. Shoot tips were analyzed by in situ hybridization to determine the earliest stage of leaf development in which AbMV is detectable. We found the first hybridization signals in the sixth to seventh leaf of the shoot tip, whereas we could not detect any viral DNA in younger leaves and meristems. These results are discussed with reference to the relation of AbMV multiplication to the cell cycle.
苘麻花叶病毒（AbMV）是一种由粉虱传播的双生病毒，具有二分体基因组。通过原位杂交，在受感染的赛洛苘麻叶片韧皮部中仅检测到AbMV DNA，且在细胞核内呈细胞内优势分布。在栅栏薄壁组织和海绵薄壁组织的细胞中未发现AbMV DNA，而这些组织显示出主要的细胞病理效应。讨论了一种假说，以解释在AbMV在韧皮部积累的情况下，却在其他组织中观察到症状这一发现。通过原位杂交分析茎尖，以确定可检测到AbMV的叶片发育最早阶段。我们在茎尖的第六至第七片叶中发现了首个杂交信号，而在较幼嫩的叶片和分生组织中未检测到任何病毒DNA。结合AbMV增殖与细胞周期的关系对这些结果进行了讨论。
